Flow cytometric characterization of cecal appendix lymphocyte subpopulations in children: a pilot study.
B-lymphocytes
Flow cytometry
NK-lymphocytes
Pediatric acute appendicitis
T-lymphocytes
Journal
Pediatric surgery international
ISSN: 1437-9813
Titre abrégé: Pediatr Surg Int
Pays: Germany
ID NLM: 8609169
Informations de publication
Date de publication:
22 Sep 2023
22 Sep 2023
Historique:
accepted:
07
09
2023
medline:
25
9
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
epublish
Résumé
Scientific literature regarding the characterization of lymphocyte subpopulations of the cecal appendix is sparse, with few precedents limited to immunohistochemical techniques. We conducted a prospective pilot study to characterize lymphocyte subpopulations of the cecal appendix in children. Participants were divided into three groups: (1) patients without histological acute appendiceal inflammation, (2) patients with histological uncomplicated acute appendicitis, and (3) patients with histological complicated acute appendicitis (gangrenous, perforated). A fresh sample of the base of the appendix was taken from all patients and a flow cytometric study was performed. Quantitative variables were compared using Kruskal-Wallis test and Mann-Whitney U test. This study included 57 patients divided into Group 1 (n = 5), Group 2 (n = 37), and Group 3 (n = 15). Median values (IQR) of the percentage of B-lymphocytes were 67.8 [66.8-68.1] in group 1, 61.15 [53.74-66.4] in group 2, and 52.1 [33-62.02] in group 3 (p = 0.02). Median values (IQR) of the percentage of NK-lymphocytes were 0.26 [0.2-0.3] in group 1, 0.55 [0.37-0.66] in group 2, and 0.84 [0.35-1.45] in group 3 (p = 0.008). Median values (IQR) of the percentage of T-lymphocytes were 31.9 [31.7-33.1] in group 1, 37.68 [32.15-45.69] in group 2, and 46.9 [37.03-67] in group 3 (p = 0.02). Pair comparisons of groups 2 and 3 also showed significant differences in the percentage of B lymphocytes (p = 0.03) and NK-lymphocytes (p = 0.02). Significant differences in lymphocyte subpopulations were identified according to the histologic grade of the cecal appendix. More specifically, a lower percentage of B-lymphocytes and a higher percentage of T- and NK-lymphocytes were observed in cases of acute appendicitis. These findings must be confirmed and their etiopathogenic, diagnostic, and prognostic implications elucidated in future studies with larger sample sizes.
Identifiants
pubmed: 37736768
doi: 10.1007/s00383-023-05558-z
pii: 10.1007/s00383-023-05558-z
pmc: PMC10516785
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
274Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2023. The Author(s).
Références
Int J Mol Sci. 2022 Dec 01;23(23):
pubmed: 36499410
Br J Surg. 2021 Dec 1;108(12):1491-1497
pubmed: 34689186
J Surg Res. 2021 Feb;258:105-112
pubmed: 33010554
Surg Today. 2000;30(12):1083-8
pubmed: 11193739
Front Cell Infect Microbiol. 2023 May 09;13:1027769
pubmed: 37228669
Front Pediatr. 2022 May 02;10:884138
pubmed: 35586830
Pediatr Surg Int. 2023 Apr 10;39(1):175
pubmed: 37038002
Indian J Pathol Microbiol. 1997 Oct;40(4):491-8
pubmed: 9444860
World J Pediatr. 2022 Dec;18(12):810-817
pubmed: 36114365
Mediators Inflamm. 2021 Oct 18;2021:4194859
pubmed: 34707461
Children (Basel). 2023 Jan 04;10(1):
pubmed: 36670658
Mediators Inflamm. 2019 Oct 28;2019:8146257
pubmed: 31772507
Indian J Pathol Microbiol. 2006 Apr;49(2):229-33
pubmed: 16933721
Int J Colorectal Dis. 2023 Apr 18;38(1):102
pubmed: 37069433
Clin Exp Immunol. 2006 Jan;143(1):117-24
pubmed: 16367942
JNMA J Nepal Med Assoc. 2015 Jan-Mar;53(197):12-7
pubmed: 26983041