Conception and Evaluation of a Library of Cleavable Mass Tags for Digital Polymers Sequencing.
Data Storage
Mass Spectrometry
Sequence-Controlled Polymers
Sequencing
Solid-Phase Chemistry
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
06 Nov 2023
06 Nov 2023
Historique:
received:
27
07
2023
medline:
22
9
2023
pubmed:
22
9
2023
entrez:
22
9
2023
Statut:
ppublish
Résumé
A library of phosphoramidite monomers containing a main-chain cleavable alkoxyamine and a side-chain substituent of variable molar mass (i.e. mass tag) was prepared in this work. These monomers can be used in automated solid-phase phosphoramidite chemistry and therefore incorporated periodically as spacers inside digitally-encoded poly(phosphodiester) chains. Consequently, the formed polymers contain tagged cleavable sites that guide their fragmentation in mass spectrometry sequencing and enhance their digital readability. The spacers were all prepared via a seven steps synthetic procedure. They were afterwards tested for the synthesis and sequencing of model digital polymers. Uniform digitally-encoded polymers were obtained as major species in all cases, even though some minor defects were sometimes detected. Furthermore, the polymers were decoded in pseudo-MS
Identifiants
pubmed: 37738223
doi: 10.1002/anie.202310801
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202310801Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-19-CE29-0015-01
Organisme : Agence Nationale de la Recherche
ID : ANR-19-CE29-0015-02
Organisme : Agence Nationale de la Recherche
ID : CSC-IGS ANR-17-EURE-0016
Informations de copyright
© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
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