Recyclable and Robust Optical Nanoprobes with Engineered Enzymes for Sustainable Serodiagnostics.

detection lanthanides nanoparticles point-of-care

Journal

Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358

Informations de publication

Date de publication:
Nov 2023
Historique:
revised: 11 09 2023
received: 06 07 2023
medline: 24 11 2023
pubmed: 22 9 2023
entrez: 22 9 2023
Statut: ppublish

Résumé

Recyclable fluorescence assays that can be stored at room temperature would greatly benefit biomedical diagnostics by bringing sustainability and cost-efficiency, especially for point-of-care serodiagnostics in developing regions. Here, a general strategy is proposed to generate recyclable fluorescent probes by using engineered enzymes with enhanced thermo-/chemo-stability, which maintains an outstanding serodiagnostic performance (accuracy >95%) after 10 times of recycling as well as after storage at elevated temperatures (37 °C for 10 days). With these three outstanding properties, recyclable fluorescent probes can be designed to detect various biomarkers of clinical importance by using different enzymes.

Identifiants

pubmed: 37738281
doi: 10.1002/adma.202306615
doi:

Substances chimiques

Fluorescent Dyes 0
Enzymes 0
Biomarkers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2306615

Subventions

Organisme : Max-Planck-Fraunhofer collaboration project
ID : Glyco3Display
Organisme : Max Planck Society
Organisme : National Natural Science Foundation of China
ID : 82001945
Organisme : Shanghai Pujiang Program
ID : 20PJ1410700
Organisme : China Scholarship Council
ID : 202008110184
Organisme : Bundesministerium für Bildung und Forschung
ID : 13XP5050A
Organisme : NWO Sector Plan for Physics and Chemistry
Organisme : EU H2020-MSCA-RISE-2017 Action program
ID : CANCER

Informations de copyright

© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.

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Auteurs

Yuxin Liu (Y)

Department of Biomolecular System, Max-Planck Institute for Colloids and Interfaces, 14476, Potsdam, Germany.
van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.
Institute of Chemistry and Biochemistry, Free University of Berlin, 14195, Berlin, Germany.

Zheng Wei (Z)

van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

Matteo Damian (M)

van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

Xingjun Zhu (X)

School of Physical Science and Technology, Shanghai Tech University, Shanghai, 201210, China.

Tanja Knaus (T)

van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

Hong Zhang (H)

van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

Francesco G Mutti (FG)

van' t Hoff Institute for Molecular Sciences, University of Amsterdam, Science Park 904, Amsterdam, 1098 XH, The Netherlands.

Felix F Loeffler (FF)

Department of Biomolecular System, Max-Planck Institute for Colloids and Interfaces, 14476, Potsdam, Germany.

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