Stem cell antigen-1

Genetic lineage tracing Myocardial fibrosis Sca1+ cells Single-cell RNA sequencing Transverse aortic constriction

Journal

Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402

Informations de publication

Date de publication:
23 Sep 2023
Historique:
received: 23 06 2023
accepted: 07 09 2023
revised: 21 08 2023
pubmed: 23 9 2023
medline: 23 9 2023
entrez: 23 9 2023
Statut: epublish

Résumé

Mesenchymal stem cells (MSCs) present in the heart cannot differentiate into cardiomyocytes, but may play a role in pathological conditions. Therefore, the aim of this study was to scrutinise the role and mechanism of MSC differentiation in vivo during heart failure. We performed single-cell RNA sequencing of total non-cardiomyocytes from murine and adult human hearts. By analysing the transcriptomes of single cells, we illustrated the dynamics of the cell landscape during the progression of heart hypertrophy, including those of stem cell antigen-1 (Sca1) Our study describes the cellular landscape of hypertrophic hearts and reveals that fibroblasts derived from Sca1

Identifiants

pubmed: 37740736
doi: 10.1007/s00018-023-04957-8
pii: 10.1007/s00018-023-04957-8
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

300

Subventions

Organisme : National Natural Science Foundation of China
ID : 82030008
Organisme : National Natural Science Foundation of China
ID : 82270409
Organisme : National Natural Science Foundation of China
ID : 31830039
Organisme : Science and Technology Program of Zhejiang Province
ID : 2023C03087

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Références

Emmons-Bell S, Johnson C, Roth G (2022) Prevalence, incidence and survival of heart failure: a systematic review. Heart 108(17):1351–1360. https://doi.org/10.1136/heartjnl-2021-320131
doi: 10.1136/heartjnl-2021-320131 pubmed: 35042750
Hall C, Gehmlich K, Denning C, Pavlovic D (2021) Complex relationship between cardiac fibroblasts and cardiomyocytes in health and disease. J Am Heart Assoc. 10(5):e019338. https://doi.org/10.1161/JAHA.120.019338 . Epub 2021 Feb 15.
Travers JG, Kamal FA, Robbins J, Yutzey KE, Blaxall BC (2016) Cardiac fibrosis: the fibroblast awakens. Circ Res 118(6):1021–1040. https://doi.org/10.1161/CIRCRESAHA.115.306565
doi: 10.1161/CIRCRESAHA.115.306565 pubmed: 26987915 pmcid: 4800485
Frangogiannis NG (2021) Cardiac fibrosis. Cardiovasc Res 117(6):1450–1488. https://doi.org/10.1093/cvr/cvaa324
doi: 10.1093/cvr/cvaa324 pubmed: 33135058
Frieler RA, Mortensen RM (2015) Immune cell and other noncardiomyocyte regulation of cardiac hypertrophy and remodeling. Circulation 131(11):1019–1030. https://doi.org/10.1161/CIRCULATIONAHA.114.008788
doi: 10.1161/CIRCULATIONAHA.114.008788 pubmed: 25779542 pmcid: 4367123
Tallquist MD (2020) Cardiac fibroblast diversity. Annu Rev Physiol 10(82):63–78. https://doi.org/10.1146/annurev-physiol-021119-034527
doi: 10.1146/annurev-physiol-021119-034527
Moore-Morris T, Guimarães-Camboa N, Banerjee I, Zambon AC, Kisseleva T, Velayoudon A, Stallcup WB, Gu Y, Dalton ND, Cedenilla M, Gomez-Amaro R, Zhou B, Brenner DA, Peterson KL, Chen J, Evans SM (2014) Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis. J Clin Invest 124(7):2921–2934. https://doi.org/10.1172/JCI74783 . (Epub 2014 Jun 17)
doi: 10.1172/JCI74783 pubmed: 24937432 pmcid: 4071409
Czubryt MP, Hale TM. Cardiac fibrosis: Pathobiology and therapeutic targets. Cell Signal. 2021 Sep;85:110066. https://doi.org/10.1016/j.cellsig.2021.110066 . Epub 2021 Jun 17.
Moore-Morris T, Guimarães-Camboa N, Yutzey KE, Pucéat M, Evans SM (2015) Cardiac fibroblasts: from development to heart failure. J Mol Med (Berl) 93(8):823–830. https://doi.org/10.1007/s00109-015-1314-y . (Epub 2015 Jul 14)
doi: 10.1007/s00109-015-1314-y pubmed: 26169532
Zeisberg EM, Tarnavski O, Zeisberg M, Dorfman AL, McMullen JR, Gustafsson E, Chandraker A, Yuan X, Pu WT, Roberts AB, Neilson EG, Sayegh MH, Izumo S, Kalluri R (2007) Endothelial-to-mesenchymal transition contributes to cardiac fibrosis. Nat Med 13(8):952–961. https://doi.org/10.1038/nm1613 . (Epub 2007 Jul 29)
doi: 10.1038/nm1613 pubmed: 17660828
Zhang L, Issa Bhaloo S, Chen T, Zhou B, Xu Q (2018) Role of resident stem cells in vessel formation and arteriosclerosis. Circ Res 122(11):1608–1624. https://doi.org/10.1161/CIRCRESAHA.118.313058
doi: 10.1161/CIRCRESAHA.118.313058 pubmed: 29798903 pmcid: 5976231
Planat-Benard V, Silvestre JS, Cousin B, André M, Nibbelink M, Tamarat R, Clergue M, Manneville C, Saillan-Barreau C, Duriez M, Tedgui A, Levy B, Pénicaud L, Casteilla L (2004) Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation 109(5):656–663. https://doi.org/10.1161/01.CIR.0000114522.38265.61 . (Epub 2004 Jan 20)
doi: 10.1161/01.CIR.0000114522.38265.61 pubmed: 14734516
Liu Q, Huang X, Zhang H, Tian X, He L, Yang R, Yan Y, Wang QD, Gillich A, Zhou B (2015) c-kit(+) cells adopt vascular endothelial but not epithelial cell fates during lung maintenance and repair. Nat Med 21(8):866–868. https://doi.org/10.1038/nm.3888 . (Epub 2015 Jul 13)
doi: 10.1038/nm.3888 pubmed: 26168292
van Berlo JH, Kanisicak O, Maillet M, Vagnozzi RJ, Karch J, Lin SC, Middleton RC, Marbán E, Molkentin JD (2014) c-kit+ cells minimally contribute cardiomyocytes to the heart. Nature 509(7500):337–341. https://doi.org/10.1038/nature13309 . (Epub 2014 May 7)
doi: 10.1038/nature13309 pubmed: 24805242 pmcid: 4127035
Gong H, Wang T, Xu Q (2021) Resident stem cells in the heart. Med Rev 1(1):10–13. https://doi.org/10.1515/mr-2021-0003
doi: 10.1515/mr-2021-0003
Holmes C, Stanford WL (2007) Concise review: stem cell antigen-1: expression, function, and enigma. Stem Cells 25(6):1339–1347. https://doi.org/10.1634/stemcells.2006-0644
doi: 10.1634/stemcells.2006-0644 pubmed: 17379763
Uchida S, De Gaspari P, Kostin S, Jenniches K, Kilic A, Izumiya Y, Shiojima I, Grosse Kreymborg K, Renz H, Walsh K, Braun T (2013) Sca1-derived cells are a source of myocardial renewal in the murine adult heart. Stem Cell Rep 1(5):397–410. https://doi.org/10.1016/j.stemcr.2013.09.004
doi: 10.1016/j.stemcr.2013.09.004
Tang J, Li Y, Huang X, He L, Zhang L, Wang H, Yu W, Pu W, Tian X, Nie Y, Hu S, Wang QD, Lui KO, Zhou B (2018) Fate mapping of Sca1+ cardiac progenitor cells in the adult mouse heart. Circulation 138(25):2967–2969. https://doi.org/10.1161/CIRCULATIONAHA.118.036210
doi: 10.1161/CIRCULATIONAHA.118.036210 pubmed: 30566021
Jolly AJ, Lu S, Strand KA, Dubner AM, Mutryn MF, Nemenoff RA, Majesky MW, Moulton KS, Weiser-Evans MCM (2022) Heterogeneous subpopulations of adventitial progenitor cells regulate vascular homeostasis and pathological vascular remodelling. Cardiovasc Res 118(6):1452–1465. https://doi.org/10.1093/cvr/cvab174
doi: 10.1093/cvr/cvab174 pubmed: 33989378
Vagnozzi RJ, Sargent MA, Lin SJ, Palpant NJ, Murry CE, Molkentin JD (2018) Genetic lineage tracing of Sca-1+ cells reveals endothelial but not myogenic contribution to the murine heart. Circulation 138(25):2931–2939. https://doi.org/10.1161/CIRCULATIONAHA.118.035210.Erratum.In:Circulation.2018Oct9;138(15):e424
doi: 10.1161/CIRCULATIONAHA.118.035210.Erratum.In:Circulation.2018Oct9;138(15):e424 pubmed: 29991486 pmcid: 6843990
Roulis M, Kaklamanos A, Schernthanner M, Bielecki P, Zhao J, Kaffe E, Frommelt LS, Qu R, Knapp MS, Henriques A, Chalkidi N, Koliaraki V, Jiao J, Brewer JR, Bacher M, Blackburn HN, Zhao X, Breyer RM, Aidinis V, Jain D, Su B, Herschman HR, Kluger Y, Kollias G, Flavell RA (2020) Paracrine orchestration of intestinal tumorigenesis by a mesenchymal niche. Nature 580(7804):524–529. https://doi.org/10.1038/s41586-020-2166-3 . (Epub 2020 Apr 1)
doi: 10.1038/s41586-020-2166-3 pubmed: 32322056 pmcid: 7490650
Hu P, Zhang D, Swenson L, Chakrabarti G, Abel ED, Litwin SE (2003) Minimally invasive aortic banding in mice: effects of altered cardiomyocyte insulin signaling during pressure overload. Am J Physiol Heart Circ Physiol 285(3):H1261-1269. https://doi.org/10.1152/ajpheart.00108.2003 . (Epub 2003 May 8)
doi: 10.1152/ajpheart.00108.2003 pubmed: 12738623
Du L, Sun X, Gong H, Wang T, Jiang L, Huang C, Xu X, Li Z, Xu H, Ma L, Li W, Chen T, Xu Q (2023) Single cell and lineage tracing studies reveal the impact of CD34+ cells on myocardial fibrosis during heart failure. Stem Cell Res Ther 14(1):33. https://doi.org/10.1186/s13287-023-03256-0
doi: 10.1186/s13287-023-03256-0 pubmed: 36805782 pmcid: 9942332
Haghverdi L, Lun ATL, Morgan MD, Marioni JC (2018) Batch effects in single-cell RNA-sequencing data are corrected by matching mutual nearest neighbors. Nat Biotechnol 36(5):421–427. https://doi.org/10.1038/nbt.4091 . (Epub 2018 Apr 2)
doi: 10.1038/nbt.4091 pubmed: 29608177 pmcid: 6152897
McGinnis CS, Murrow LM, Gartner ZJ (2019) DoubletFinder: doublet detection in single-cell RNA sequencing data using artificial nearest neighbors. Cell Syst 8(4):329-337.e4. https://doi.org/10.1016/j.cels.2019.03.003 . (Epub 2019 Apr 3)
doi: 10.1016/j.cels.2019.03.003 pubmed: 30954475 pmcid: 6853612
Stuart T, Butler A, Hoffman P, Hafemeister C, Papalexi E, Mauck WM 3rd, Hao Y, Stoeckius M, Smibert P, Satija R (2019) Comprehensive integration of single-cell data. Cell 177(7):1888-1902.e21. https://doi.org/10.1016/j.cell.2019.05.031 . (Epub 2019 Jun 6)
doi: 10.1016/j.cell.2019.05.031 pubmed: 31178118 pmcid: 6687398
Buechler MB, Pradhan RN, Krishnamurty AT, Cox C, Calviello AK, Wang AW, Yang YA, Tam L, Caothien R, Roose-Girma M, Modrusan Z, Arron JR, Bourgon R, Müller S, Turley SJ (2021) Cross-tissue organization of the fibroblast lineage. Nature 593(7860):575–579. https://doi.org/10.1038/s41586-021-03549-5 . (Epub 2021 May 12)
doi: 10.1038/s41586-021-03549-5 pubmed: 33981032
Pu X, Zhu P, Zhou X, He Y, Wu H, Du L, Gong H, Sun X, Chen T, Zhu J, Xu Q, Zhang H (2022) CD34+ cell atlas of main organs implicates its impact on fibrosis. Cell Mol Life Sci 79(11):576. https://doi.org/10.1007/s00018-022-04606-6
doi: 10.1007/s00018-022-04606-6 pubmed: 36315271
Qiu X, Hill A, Packer J, Lin D, Ma YA, Trapnell C (2017) Single-cell mRNA quantification and differential analysis with Census. Nat Methods 14(3):309–315. https://doi.org/10.1038/nmeth.4150 . (Epub 2017 Jan 23)
doi: 10.1038/nmeth.4150 pubmed: 28114287 pmcid: 5330805
Trapnell C, Cacchiarelli D, Grimsby J, Pokharel P, Li S, Morse M, Lennon NJ, Livak KJ, Mikkelsen TS, Rinn JL (2014) The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nat Biotechnol 32(4):381–386. https://doi.org/10.1038/nbt.2859 . (Epub 2014 Mar 23)
doi: 10.1038/nbt.2859 pubmed: 24658644 pmcid: 4122333
Plummer NW, Ungewitter EK, Smith KG, Yao HH, Jensen P. A new mouse line for cell ablation by diphtheria toxin subunit A controlled by a Cre-dependent FLEx switch. Genesis. 2017 Oct;55(10): https://doi.org/10.1002/dvg.23067 . doi: https://doi.org/10.1002/dvg.23067 . Epub 2017 Sep 19.
Lee CH, Shah B, Moioli EK, Mao JJ (2010) CTGF directs fibroblast differentiation from human mesenchymal stem/stromal cells and defines connective tissue healing in a rodent injury model. J Clin Invest. 120(9):3340–3349. https://doi.org/10.1172/JCI43230 . Epub 2010 Aug 2. Erratum in: J Clin Invest. 2015 Oct 1;125(10):3992.
Plikus MV, Wang X, Sinha S, Forte E, Thompson SM, Herzog EL, Driskell RR, Rosenthal N, Biernaskie J, Horsley V (2021) Fibroblasts: Origins, definitions, and functions in health and disease. Cell 184(15):3852–3872. https://doi.org/10.1016/j.cell.2021.06.024
doi: 10.1016/j.cell.2021.06.024 pubmed: 34297930 pmcid: 8566693
Soliman H, Theret M, Scott W, Hill L, Underhill TM, Hinz B, Rossi FMV (2021) Multipotent stromal cells: one name, multiple identities. Cell Stem Cell 28(10):1690–1707. https://doi.org/10.1016/j.stem.2021.09.001
doi: 10.1016/j.stem.2021.09.001 pubmed: 34624231
Aghajanian H, Kimura T, Rurik JG, Hancock AS, Leibowitz MS, Li L, Scholler J, Monslow J, Lo A, Han W, Wang T, Bedi K, Morley MP, Linares Saldana RA, Bolar NA, McDaid K, Assenmacher CA, Smith CL, Wirth D, June CH, Margulies KB, Jain R, Puré E, Albelda SM, Epstein JA (2019) Targeting cardiac fibrosis with engineered T cells. Nature 573(7774):430–433. https://doi.org/10.1038/s41586-019-1546-z . (Epub 2019 Sep 11)
doi: 10.1038/s41586-019-1546-z pubmed: 31511695 pmcid: 6752964
van Amerongen MJ, Bou-Gharios G, Popa E, van Ark J, Petersen AH, van Dam GM, van Luyn MJ, Harmsen MC (2008) Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. J Pathol 214(3):377–386. https://doi.org/10.1002/path.2281
doi: 10.1002/path.2281 pubmed: 18095257
Tallquist MD, Molkentin JD (2017) Redefining the identity of cardiac fibroblasts. Nat Rev Cardiol 14(8):484–491. https://doi.org/10.1038/nrcardio.2017.57 . (Epub 2017 Apr 24)
doi: 10.1038/nrcardio.2017.57 pubmed: 28436487 pmcid: 6329009
Torsney E, Xu Q (2011) Resident vascular progenitor cells. J Mol Cell Cardiol 50(2):304–311. https://doi.org/10.1016/j.yjmcc.2010.09.006
doi: 10.1016/j.yjmcc.2010.09.006 pubmed: 20850452
Oh H, Chi X, Bradfute SB, Mishina Y, Pocius J, Michael LH, Behringer RR, Schwartz RJ, Entman ML, Schneider MD (2004) Cardiac muscle plasticity in adult and embryo by heart-derived progenitor cells. Ann N Y Acad Sci 1015:182–189. https://doi.org/10.1196/annals.1302.015
doi: 10.1196/annals.1302.015 pubmed: 15201159
Ni Z, Lyu L, Gong H, Du L, Wen Z, Jiang H, Yang H, Hu Y, Zhang B, Xu Q, Guo X, Chen T (2023) Multilineage commitment of Sca-1+ cells in reshaping vein grafts. Theranostics 13(7):2154–2175. https://doi.org/10.7150/thno.77735
doi: 10.7150/thno.77735 pubmed: 37153747 pmcid: 10157743
Tang J, Wang H, Huang X, Li F, Zhu H, Li Y, He L, Zhang H, Pu W, Liu K, Zhao H, Bentzon JF, Yu Y, Ji Y, Nie Y, Tian X, Zhang L, Gao D, Zhou B (2020) Arterial Sca1
doi: 10.1016/j.stem.2019.11.010 pubmed: 31883835
Ito CY, Li CY, Bernstein A, Dick JE, Stanford WL (2003) Hematopoietic stem cell and progenitor defects in Sca-1/Ly-6A-null mice. Blood 101(2):517–523. https://doi.org/10.1182/blood-2002-06-1918 . (Epub 2002 Aug 29)
doi: 10.1182/blood-2002-06-1918 pubmed: 12393491
Morcos MNF, Schoedel KB, Hoppe A, Behrendt R, Basak O, Clevers HC, Roers A, Gerbaulet A (2017) SCA-1 expression level identifies quiescent hematopoietic stem and progenitor cells. Stem Cell Reports 8(6):1472–1478. https://doi.org/10.1016/j.stemcr.2017.04.012 . (Epub 2017 May 11)
doi: 10.1016/j.stemcr.2017.04.012 pubmed: 28506535 pmcid: 5469944
assone NM, Li B, Patel MS, Devine MY, Firmiss PR, Gould AD, Kochan KS, Stubbee RA, Bowen DK, Dettman RW, Gong EM (2019) Stem cell antigen/Ly6a protects against bladder fibrosis in mice. Am J Physiol Renal Physiol. 317(6):F1503-F1512. https://doi.org/10.1152/ajprenal.00160.2019 . Epub 2019 Sep 18.
Walter DH, Haendeler J, Reinhold J, Rochwalsky U, Seeger F, Honold J, Hoffmann J, Urbich C, Lehmann R, Arenzana-Seisdesdos F, Aicher A, Heeschen C, Fichtlscherer S, Zeiher AM, Dimmeler S (2005) Impaired CXCR4 signaling contributes to the reduced neovascularization capacity of endothelial progenitor cells from patients with coronary artery disease. Circ Res 97(11):1142–1151. https://doi.org/10.1161/01.RES.0000193596.94936.2c . (Epub 2005 Oct 27)
doi: 10.1161/01.RES.0000193596.94936.2c pubmed: 16254213
Zhou H, Bian ZY, Zong J, Deng W, Yan L, Shen DF, Guo H, Dai J, Yuan Y, Zhang R, Lin YF, Hu X, Li H, Tang QZ (2012) Stem cell antigen 1 protects against cardiac hypertrophy and fibrosis after pressure overload. Hypertension 60(3):802–809. https://doi.org/10.1161/HYPERTENSIONAHA.112.198895 . (Epub 2012 Jul 30)
doi: 10.1161/HYPERTENSIONAHA.112.198895 pubmed: 22851736
Deng J, Ni Z, Gu W, Chen Q, Nowak WN, Chen T, Issa Bhaloo S, Zhang Z, Hu Y, Zhou B, Zhang L, Xu Q (2020) Single-cell gene profiling and lineage tracing analyses revealed novel mechanisms of endothelial repair by progenitors. Cell Mol Life Sci 77(24):5299–5320. https://doi.org/10.1007/s00018-020-03480-4 . (Epub 2020 Mar 13)
doi: 10.1007/s00018-020-03480-4 pubmed: 32166394
Dong W, Zhao Y, Wen D, Lin Y, Zeng C, Gu J, Liao F, Li R, Zhang X, Wang D, Cai W, Duan J (2022) Wnt4 is crucial for cardiac repair by regulating mesenchymal-endothelial transition via the phospho-JNK/JNK. Theranostics 12(9):4110–4126. https://doi.org/10.7150/thno.71392
doi: 10.7150/thno.71392 pubmed: 35673578 pmcid: 9169355
Haybar H, Khodadi E, Shahrabi S (2019) Wnt/β-catenin in ischemic myocardium: interactions and signaling pathways as a therapeutic target. Heart Fail Rev 24(3):411–419. https://doi.org/10.1007/s10741-018-9759-z
doi: 10.1007/s10741-018-9759-z pubmed: 30539334
Brade T, Männer J, Kühl M (2006) The role of Wnt signalling in cardiac development and tissue remodelling in the mature heart. Cardiovasc Res 72(2):198–209. https://doi.org/10.1016/j.cardiores.2006.06.025 . (Epub 2006 Jun 29)
doi: 10.1016/j.cardiores.2006.06.025 pubmed: 16860783
van de Rijn M, Heimfeld S, Spangrude GJ, Weissman IL (1989) Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family. Proc Natl Acad Sci U S A 86(12):4634–4638. https://doi.org/10.1073/pnas.86.12.4634
doi: 10.1073/pnas.86.12.4634 pubmed: 2660142 pmcid: 287325

Auteurs

Tingting Tao (T)

Department of Cardiovascular Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Luping Du (L)

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Peng Teng (P)

Department of Cardiovascular Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Yan Guo (Y)

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Xuyang Wang (X)

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Yanhua Hu (Y)

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Haige Zhao (H)

Department of Cardiovascular Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China.

Qingbo Xu (Q)

Department of Cardiology, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China. qingbo_xu@zju.edu.cn.

Liang Ma (L)

Department of Cardiovascular Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, China. ml1402@zju.edu.cn.

Classifications MeSH