Key topographic parameters driving surface adhesion of Porphyromonas gingivalis.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
23 09 2023
Historique:
received: 17 05 2023
accepted: 09 09 2023
medline: 20 11 2023
pubmed: 24 9 2023
entrez: 23 9 2023
Statut: epublish

Résumé

Dental implant failure is primarily due to peri-implantitis, a consequence of bacterial biofilm formation. Bacterial adhesion is strongly linked to micro-/nano-topographies of a surface; thus an assessment of surface texture parameters is essential to understand bacterial adhesion. In this study, mirror polished titanium samples (Ti6Al4V) were irradiated with a femtosecond laser (fs-L) at a wavelength of 1030 nm (infrared) with variable laser parameters (laser beam polarization, number, spacing and organization of the impacts). Images of 3-D topographies were obtained by focal variation microscopy and analyzed with MountainsMap software to measure surface parameters. From bacteria associated with peri-implantitis, we selected Porphyromonas gingivalis to evaluate its adhesion on Ti6Al4V surfaces in an in vitro study. Correlations between various surface parameters and P. gingivalis adhesion were investigated. We discovered that Sa value, a common measure of surface roughness, was not sufficient in describing the complexity of these fs-L treated surfaces and their bacterial interaction. We found that Sku, density and mean depths of the furrows, were the most accurate parameters for this purpose. These results provide important information that could help anticipate the bacterial adhesive properties of a surface based on its topographic parameters, thus the development of promising laser designed biofunctional implants.

Identifiants

pubmed: 37741851
doi: 10.1038/s41598-023-42387-5
pii: 10.1038/s41598-023-42387-5
pmc: PMC10518006
doi:

Substances chimiques

titanium alloy (TiAl6V4) 12743-70-3
Titanium D1JT611TNE
Dental Implants 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15893

Informations de copyright

© 2023. The Author(s).

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Auteurs

Steve Papa (S)

INSERM, SAINBIOSE U1059, Mines Saint-Etienne, Université Jean Monnet Saint-Étienne, 42023, Saint-Étienne, France. steve.papa@univ-st-etienne.fr.

Mathieu Maalouf (M)

INSERM, SAINBIOSE U1059, Mines Saint-Etienne, Université Jean Monnet Saint-Étienne, 42023, Saint-Étienne, France.

Pierre Claudel (P)

GIE Manutech-USD, 20 Rue Benoît Lauras, 42000, Saint-Étienne, France.

Xxx Sedao (X)

GIE Manutech-USD, 20 Rue Benoît Lauras, 42000, Saint-Étienne, France.
Laboratory Hubert Curien, UMR 5516 CNRS, Jean Monnet University, University of Lyon, 42000, Saint-Étienne, France.

Yoan Di Maio (Y)

GIE Manutech-USD, 20 Rue Benoît Lauras, 42000, Saint-Étienne, France.

Hind Hamzeh-Cognasse (H)

INSERM, SAINBIOSE U1059, Mines Saint-Etienne, Université Jean Monnet Saint-Étienne, 42023, Saint-Étienne, France.

Mireille Thomas (M)

INSERM, SAINBIOSE U1059, Mines Saint-Etienne, Université Jean Monnet Saint-Étienne, 42023, Saint-Étienne, France.

Alain Guignandon (A)

INSERM, SAINBIOSE U1059, Mines Saint-Etienne, Université Jean Monnet Saint-Étienne, 42023, Saint-Étienne, France.

Virginie Dumas (V)

Ecole Centrale de Lyon, CNRS, ENTPE, LTDS, UMR5513, ENISE, Univ Lyon, 42023, Saint-Étienne, France.

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Classifications MeSH