Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage.
Bovine mastitis
Endolysin
Jumbo phages
Staphylococcus aureus
VAPGH
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
03
04
2023
accepted:
21
08
2023
revised:
03
04
2023
medline:
13
11
2023
pubmed:
24
9
2023
entrez:
23
9
2023
Statut:
ppublish
Résumé
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. Among the different strategies to tackle S. aureus infection in livestock, bacteriophages have been thoroughly investigated in the last decades; however, few specimens of the so-called jumbo phages capable of infecting S. aureus have been described. Herein, we report the biological, genomic, and structural proteomic features of the jumbo phage vB_SauM-UFV_DC4 (DC4). DC4 exhibited a remarkable killing activity against S. aureus isolated from the veterinary environment and stability at alkaline conditions (pH 4 to 12). The complete genome of DC4 is 263,185 bp (GC content: 25%), encodes 263 predicted CDSs (80% without an assigned function), 1 tRNA (Phe-tRNA), multisubunit RNA polymerase, and an RNA-dependent DNA polymerase. Moreover, comparative analysis revealed that DC4 can be considered a new viral species belonging to a new genus DC4 and showed a similar set of lytic proteins and depolymerase activity with closely related jumbo phages. The characterization of a new S. aureus jumbo phage increases our understanding of the diversity of this group and provides insights into the biotechnological potential of these viruses. KEY POINTS: • vB_SauM-UFV_DC4 is a new viral species belonging to a new genus within the class Caudoviricetes. • vB_SauM-UFV_DC4 carries a set of RNA polymerase subunits and an RNA-directed DNA polymerase. • vB_SauM-UFV_DC4 and closely related jumbo phages showed a similar set of lytic proteins.
Identifiants
pubmed: 37741937
doi: 10.1007/s00253-023-12743-6
pii: 10.1007/s00253-023-12743-6
pmc: PMC10638138
doi:
Substances chimiques
DNA-Directed RNA Polymerases
EC 2.7.7.6
RNA, Transfer
9014-25-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7231-7250Informations de copyright
© 2023. The Author(s).
Références
J Virol. 2021 Sep 9;95(19):e0239120
pubmed: 34287047
Bioinformatics. 2008 Aug 1;24(15):1662-8
pubmed: 18474507
Front Microbiol. 2021 Sep 14;12:723834
pubmed: 34594314
Viruses. 2021 Jan 05;13(1):
pubmed: 33466489
Viruses. 2020 Sep 23;12(10):
pubmed: 32977622
BMC Vet Res. 2022 Mar 24;18(1):115
pubmed: 35331225
Appl Microbiol Biotechnol. 2018 Mar;102(6):2563-2581
pubmed: 29442169
Sci Rep. 2016 Oct 04;6:34338
pubmed: 27698408
Viruses. 2018 Apr 07;10(4):
pubmed: 29642449
Methods Mol Biol. 2018;1681:41-47
pubmed: 29134585
Nat Microbiol. 2020 Jan;5(1):48-55
pubmed: 31819217
Front Microbiol. 2021 Apr 20;12:660351
pubmed: 33959116
FEMS Microbiol Rev. 2006 May;30(3):321-81
pubmed: 16594962
Front Microbiol. 2019 Jul 23;10:1533
pubmed: 31428059
Nat Commun. 2018 Nov 14;9(1):4781
pubmed: 30429469
Nat Commun. 2018 Nov 30;9(1):5114
pubmed: 30504855
Viruses. 2019 Nov 23;11(12):
pubmed: 31771160
ISME J. 2022 Jun;16(6):1657-1667
pubmed: 35260829
Clin Microbiol Rev. 2021 Feb 10;34(2):
pubmed: 33568553
mBio. 2018 Jan 23;9(1):
pubmed: 29362234
Pathog Dis. 2014 Apr;70(3):231-9
pubmed: 24453168
Front Microbiol. 2018 Oct 08;9:2419
pubmed: 30349525
PLoS One. 2010 Jun 25;5(6):e11147
pubmed: 20593022
J Bacteriol. 2004 Mar;186(5):1503-17
pubmed: 14973019
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W244-8
pubmed: 15980461
Clin Microbiol Infect. 2022 Sep;28(9):1193-1202
pubmed: 35339675
Nucleic Acids Res. 2021 Jan 8;49(D1):D192-D200
pubmed: 33211869
Nucleic Acids Res. 2017 Jan 4;45(D1):D491-D498
pubmed: 27789703
Methods Mol Biol. 2009;502:239-51
pubmed: 19082560
Nucleic Acids Res. 2021 Jan 8;49(D1):D344-D354
pubmed: 33156333
Theor Popul Biol. 2002 Jun;61(4):471-80
pubmed: 12167366
Nat Methods. 2011 Sep 29;8(10):785-6
pubmed: 21959131
PLoS One. 2022 Apr 14;17(4):e0266891
pubmed: 35421196
Viruses. 2018 Jun 07;10(6):
pubmed: 29875339
Adv Sci (Weinh). 2019 Dec 05;7(1):1901872
pubmed: 31921562
Vet Microbiol. 2018 May;218:45-51
pubmed: 29685220
Bacteriophage. 2016 Aug 5;6(3):e1219441
pubmed: 27738556
J Struct Biol. 2006 Oct;156(1):200-9
pubmed: 16677824
Viruses. 2021 Mar 18;13(3):
pubmed: 33803862
Viruses. 2014 Jul 01;6(7):2551-70
pubmed: 24988520
ISME J. 2014 Sep;8(9):1949-52
pubmed: 24599069
J Bacteriol. 2020 Dec 18;203(2):
pubmed: 32868402
Microbiol Resour Announc. 2019 May 30;8(22):
pubmed: 31147429
Nucleic Acids Res. 2017 Jun 2;45(10):5958-5967
pubmed: 28402520
Front Microbiol. 2019 Nov 28;10:2772
pubmed: 31849908
Anim Microbiome. 2022 Apr 8;4(1):26
pubmed: 35395785
PeerJ. 2021 May 6;9:e11396
pubmed: 33996289
Front Microbiol. 2016 Oct 13;7:1603
pubmed: 27790200
Viruses. 2020 Nov 06;12(11):
pubmed: 33172115
Bioinformatics. 2017 Aug 01;33(15):2379-2380
pubmed: 28379287
Nucleic Acids Res. 2019 Jul 2;47(W1):W52-W58
pubmed: 31053848
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296
pubmed: 33885785
Microbiol Resour Announc. 2023 Jan 24;12(1):e0108622
pubmed: 36598273
PLoS One. 2013 Jun 18;8(6):e66654
pubmed: 23824136
Annu Rev Genet. 2000;34:439-456
pubmed: 11092834
NPJ Biofilms Microbiomes. 2021 Apr 22;7(1):39
pubmed: 33888725
Viruses. 2016 May 04;8(5):
pubmed: 27153081
J Antimicrob Chemother. 2020 Dec 1;75(12):3491-3500
pubmed: 32780112
J Virol. 2014 Sep;88(18):10501-10
pubmed: 24965474
BMC Genomics. 2011 Aug 08;12:402
pubmed: 21824423
Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents
pubmed: 12626685
Environ Microbiol. 2017 Dec;19(12):5060-5077
pubmed: 29076652
Antibiotics (Basel). 2019 Jul 25;8(3):
pubmed: 31349628
PLoS Comput Biol. 2011 Oct;7(10):e1002195
pubmed: 22039361
Virology. 2016 Aug;495:185-96
pubmed: 27236306
Sci Rep. 2020 Mar 26;10(1):5520
pubmed: 32218514
Infect Drug Resist. 2021 Nov 30;14:5041-5055
pubmed: 34876823
PLoS One. 2011 Apr 26;6(4):e18673
pubmed: 21541311
Bioinformatics. 2017 Nov 01;33(21):3396-3404
pubmed: 29036289
Phage (New Rochelle). 2021 Dec 1;2(4):214-223
pubmed: 36159887
Nucleic Acids Res. 2004 Jan 02;32(1):11-6
pubmed: 14704338
J Mol Biol. 2001 Jan 19;305(3):567-80
pubmed: 11152613
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W429-32
pubmed: 17483518
Microbiol Spectr. 2019 May;7(3):
pubmed: 31124433
Cell Rep. 2021 Jun 8;35(10):109214
pubmed: 34107245
Nucleic Acids Res. 2016 Jan 4;44(D1):D279-85
pubmed: 26673716
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
pubmed: 9254694
Biotechniques. 2000 Jun;28(6):1102, 1104
pubmed: 10868275
Appl Microbiol Biotechnol. 2017 Apr;101(8):3103-3119
pubmed: 28337580
Nucleic Acids Res. 2023 Jan 6;51(D1):D418-D427
pubmed: 36350672
Antibiotics (Basel). 2021 Dec 06;10(12):
pubmed: 34943709
Sci Rep. 2018 May 1;8(1):6845
pubmed: 29717158
Nucleic Acids Res. 2016 Jul 8;44(W1):W54-7
pubmed: 27174935
Front Microbiol. 2017 Mar 14;8:403
pubmed: 28352259
Front Microbiol. 2021 Mar 08;12:622755
pubmed: 33763042
Nucleic Acids Res. 2017 Jul 3;45(W1):W12-W16
pubmed: 28521017
Front Microbiol. 2022 Apr 28;13:856473
pubmed: 35572667
Appl Environ Microbiol. 2012 Apr;78(7):2297-305
pubmed: 22286996
Front Pharmacol. 2022 Jun 02;13:838092
pubmed: 35721221
Microb Genom. 2018 Apr;4(4):
pubmed: 29633935
Vet Microbiol. 2016 Aug 15;191:65-71
pubmed: 27374909
Front Microbiol. 2019 Jul 02;10:1491
pubmed: 31312191
PLoS One. 2015 Mar 11;10(3):e0118557
pubmed: 25761060
FEMS Microbiol Lett. 2006 Dec;265(1):133-9
pubmed: 17054440
Bioinformatics. 2008 Dec 15;24(24):2928-9
pubmed: 18945683
Bioinformatics. 2014 Jul 15;30(14):2068-9
pubmed: 24642063
Viruses. 2020 Dec 25;13(1):
pubmed: 33375688