Highly efficient Agrobacterium rhizogenes-mediated transformation for functional analysis in woodland strawberry.
Agrobacterium rhizogenes
Hairy root
Rosaceae
Woodland strawberry
Journal
Plant methods
ISSN: 1746-4811
Titre abrégé: Plant Methods
Pays: England
ID NLM: 101245798
Informations de publication
Date de publication:
23 Sep 2023
23 Sep 2023
Historique:
received:
03
06
2023
accepted:
08
09
2023
medline:
24
9
2023
pubmed:
24
9
2023
entrez:
23
9
2023
Statut:
epublish
Résumé
The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root transformation is an alternative for exploring gene functions, especially the genes specifically expressed in roots. However, the hairy root transformation has not been established in strawberry. Here, we described an efficient and rapid hairy root transgenic system for strawberry using A. rhizogenes. Strain of A. rhizogenes MSU440 or C58C1 was the most suitable for hairy root transformation. The transformation efficiency was highest when tissues contained hypocotyls as explants. The optimal procedure involves A. rhizogenes at an optical density (OD We established a rapid and efficient hairy root transformation in strawberry by optimizing parameters, which was adequate for promoter analysis and functional characterization of candidate genes in strawberry and other rosaceous plants.
Sections du résumé
BACKGROUND
BACKGROUND
The diploid woodland strawberry (Fragaria vesca) is an excellent model plant for investigating economically significant traits and several genetic resources within the Rosaceae family. Agrobacterium rhizogenes-mediated hairy root transformation is an alternative for exploring gene functions, especially the genes specifically expressed in roots. However, the hairy root transformation has not been established in strawberry.
RESULTS
RESULTS
Here, we described an efficient and rapid hairy root transgenic system for strawberry using A. rhizogenes. Strain of A. rhizogenes MSU440 or C58C1 was the most suitable for hairy root transformation. The transformation efficiency was highest when tissues contained hypocotyls as explants. The optimal procedure involves A. rhizogenes at an optical density (OD
CONCLUSION
CONCLUSIONS
We established a rapid and efficient hairy root transformation in strawberry by optimizing parameters, which was adequate for promoter analysis and functional characterization of candidate genes in strawberry and other rosaceous plants.
Identifiants
pubmed: 37742022
doi: 10.1186/s13007-023-01078-y
pii: 10.1186/s13007-023-01078-y
pmc: PMC10517450
doi:
Types de publication
Journal Article
Langues
eng
Pagination
99Subventions
Organisme : National Natural Science Foundation of China
ID : 32160699 and 32060587
Organisme : Joint Fund of the National Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province
ID : U1812401
Organisme : Natural Science Foundation of Guizhou Province
ID : QKHJC-ZK[2022]ZD032
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
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