Glycolipids from Sargassum filipendula, a Natural Alternative for Overcoming ABC Transporter-Mediated MDR in Cancer.


Journal

Chemistry & biodiversity
ISSN: 1612-1880
Titre abrégé: Chem Biodivers
Pays: Switzerland
ID NLM: 101197449

Informations de publication

Date de publication:
Nov 2023
Historique:
received: 19 07 2023
accepted: 25 09 2023
medline: 29 11 2023
pubmed: 25 9 2023
entrez: 25 9 2023
Statut: ppublish

Résumé

Chemotherapy is a widely used strategy to treat cancer, a disease that causes millions of deaths each year. However, its efficacy is reduced by the overexpression of ABC transporters, which are proteins that expel the drugs used in chemotherapy and involved in the multidrug resistance (MDR). Glycolipids have been identified as potential inhibitors of ABC transporters. Algae of the genus Sargassum contain high levels of glycolipids, making them a promising therapeutic alternative against the MDR phenotype. Sargassum filipendula glycolipids were obtained by exhaustive maceration with chloroform/methanol, purified by column and thin layer chromatography, and then characterized by FTIR, NMR, and LC-MS. Cell viability by PI labeling and inhibition of ABC transporters were analyzed by flow cytometry. Assessment of resistance reversal was determined by MTT assay. Ten sulfoquinovosylglycerol-type compounds were found, and six of them are reported for the first time. In particular, moiety 4 (GL-4) showed strong and moderate inhibitory activity against ABCC1 and ABCB1 transporters respectively. Treatment of GL-4 in combination with the antineoplastic drug vincristine sensitized Lucena-1 cell model to drug and reversed the MDR phenotype. This is the first report of glycolipids isolated from S. filipendula capable of inhibiting ABC transporters and thus overcoming acquired drug resistance.

Identifiants

pubmed: 37747792
doi: 10.1002/cbdv.202301058
doi:

Substances chimiques

ATP-Binding Cassette Transporters 0
Forssman glycolipid 60267-39-2
Antineoplastic Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202301058

Subventions

Organisme : Universidad de Antioquia
Organisme : Institute of Chemistry of the University of Antioquia
Organisme : CODI of the Universidad de Antioquia
ID : 2019-25210
Organisme : Glycobiology Laboratory/IBCCF/UFRJ
Organisme : CNPq
Organisme : FAPERJ
Organisme : Cancer Foundation/Brazil

Informations de copyright

© 2023 Wiley-VHCA AG, Zurich, Switzerland.

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Auteurs

Kelly Muñoz-Losada (K)

Grupo de Investigación en Compuestos Funcionales, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, 050010, Colombia.

Kelli Monteiro Da Costa (KM)

Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, 21941-902, Brasil.

Tatiana Muñoz-Castiblanco (T)

Grupo de Investigación en Compuestos Funcionales, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, 050010, Colombia.

Juan Camilo Mejía-Giraldo (JC)

Grupo de Investigación en Compuestos Funcionales, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, 050010, Colombia.
Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, 050010, Colombia.

José Osvaldo Previato (JO)

Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, 21941-902, Brasil.

Lucia Mendonça-Previato (L)

Laboratório de Glicobiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, 21941-902, Brasil.

Miguel Ángel Puertas-Mejía (MÁ)

Grupo de Investigación en Compuestos Funcionales, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, 050010, Colombia.

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