Unraveling the kinetics and pharmacology of human PepT1 using solid supported membrane-based electrophysiology.
Biosensor
High‐throughput screening (HTS)
Membrane transport
Peptide Transporter 1 (PepT1)
Pharmacology
Solid Supported Membrane-based Electrophysiology (SSME)
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Feb 2024
Feb 2024
Historique:
received:
17
07
2023
revised:
14
09
2023
accepted:
15
09
2023
medline:
27
11
2023
pubmed:
26
9
2023
entrez:
25
9
2023
Statut:
ppublish
Résumé
The human Peptide Transporter 1 (hPepT1) is known for its broad substrate specificity and its ability to transport (pro-)drugs. Here, we present an in-depth comprehensive study of hPepT1 and its interactions with various substrates via solid supported membrane-based electrophysiology (SSME). Using hPepT1-containing vesicles, we could not identify any peptide induced pre-steady-state currents, indicating that the recorded peak currents reflect steady-state transport. Electrogenic co-transport of H
Identifiants
pubmed: 37748262
pii: S1567-5394(23)00210-4
doi: 10.1016/j.bioelechem.2023.108573
pii:
doi:
Substances chimiques
Symporters
0
Peptide Transporter 1
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108573Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Authors AB, MB and NF are employed by Nanion Technologies GmbH, the developer of the SURFE(2)R instruments.