Potential involvement of IL-32 in cell-to-cell communication between macrophages and hepatoblastoma.
Cell–cell communication
Hepatoblastoma
IL-32
IL-6
Macrophage
PD-L1
Journal
Pediatric surgery international
ISSN: 1437-9813
Titre abrégé: Pediatr Surg Int
Pays: Germany
ID NLM: 8609169
Informations de publication
Date de publication:
26 Sep 2023
26 Sep 2023
Historique:
accepted:
31
08
2023
medline:
27
9
2023
pubmed:
26
9
2023
entrez:
26
9
2023
Statut:
epublish
Résumé
This study investigated the expression of interleukin 32 (IL-32) in hepatoblastoma, the most common primary pediatric liver tumor, and its possible roles in tumorigenesis. IL-32 expression was investigated in two hepatoblastoma cell lines (Hep G2 and HuH 6) in the steady state and after co-culture with macrophages by RNA-seq analysis and RT-qPCR, and after stimulation with chemotherapy. Cultured macrophages were stimulated by IL-32 isoforms followed by RT-qPCR and western blot analysis. IL-32 immunohistochemical staining (IHC) was performed using specimens from 21 hepatoblastoma patients. Clustering analysis was also performed using scRNA-seq data downloaded from Gene Expression Omnibus. The IL-32 gene is expressed by hepatoblastoma cell lines; expression is upregulated by paracrine cell-cell communication with macrophages, also by carboplatin and etoposide. IL-32 causes protumor activation of macrophages with upregulation of PD-L1, IDO-1, IL-6, and IL-10. In the patient pool, IHC was positive only in 48% of cases. However, in the downloaded dataset, IL-32 gene expression was negative. IL-32 was detected in hepatoblastoma cell lines, but not in all hepatoblastoma patients. We hypothesized that stimulation such as chemotherapy might induce expression of IL-32, which might be a critical mediator of chemoresistance in hepatoblastoma through inducing protumor activation in macrophages.
Identifiants
pubmed: 37751001
doi: 10.1007/s00383-023-05557-0
pii: 10.1007/s00383-023-05557-0
doi:
Substances chimiques
Interleukins
0
IL32 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
275Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology of Japan
ID : 20H03459
Organisme : Ministry of Education, Culture, Sports, Science and Technology of Japan
ID : 21K08622
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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