Machine learning for cross-scale microscopy of viruses.

CP: Microbiology and CP: Imaging SARS-CoV-2 adenovirus tracking and trafficking artificial intelligence deep learning electron microscopy fluorescence super-resolution microscopy herpes simplex virus human immunodeficiency virus influenza virus machine learning nanoparticle

Journal

Cell reports methods
ISSN: 2667-2375
Titre abrégé: Cell Rep Methods
Pays: United States
ID NLM: 9918227360606676

Informations de publication

Date de publication:
25 09 2023
Historique:
received: 06 02 2023
revised: 05 06 2023
accepted: 20 07 2023
medline: 28 9 2023
pubmed: 27 9 2023
entrez: 26 9 2023
Statut: ppublish

Résumé

Despite advances in virological sciences and antiviral research, viruses continue to emerge, circulate, and threaten public health. We still lack a comprehensive understanding of how cells and individuals remain susceptible to infectious agents. This deficiency is in part due to the complexity of viruses, including the cell states controlling virus-host interactions. Microscopy samples distinct cellular infection stages in a multi-parametric, time-resolved manner at molecular resolution and is increasingly enhanced by machine learning and deep learning. Here we discuss how state-of-the-art artificial intelligence (AI) augments light and electron microscopy and advances virological research of cells. We describe current procedures for image denoising, object segmentation, tracking, classification, and super-resolution and showcase examples of how AI has improved the acquisition and analyses of microscopy data. The power of AI-enhanced microscopy will continue to help unravel virus infection mechanisms, develop antiviral agents, and improve viral vectors.

Identifiants

pubmed: 37751685
pii: S2667-2375(23)00201-1
doi: 10.1016/j.crmeth.2023.100557
pmc: PMC10545915
pii:
doi:

Substances chimiques

Antiviral Agents 0

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

100557

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Anthony Petkidis (A)

Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. Electronic address: anthony.petkidis@uzh.ch.

Vardan Andriasyan (V)

Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.

Urs F Greber (UF)

Department of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. Electronic address: urs.greber@mls.uzh.ch.

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