[Application of Rapid HE Staining in Cytological Rapid On-site Evaluation of Peripheral Lung Cancer Needle Biopsy].

BACKGROUND

Rapid on-site evaluation (ROSE) is a technique used for simultaneous evaluation of biopsy specimens through rapid cytology staining. Diff-Quik (DQ) staining is the most commonly employed method for cytological rapid on-site evaluation (C-ROSE). However, the utilization of DQ staining for on-site cytological interpretation remains uncommon among pathologists in China, posing challenges to the implementation of C-ROSE. This study aims to assess the application of rapid hematoxylin-eosin (HE) staining and DQ staining for C-ROSE during percutaneous needle biopsy of peripheral lung cancer and evaluate the value of rapid HE staining in C-ROSE.

METHODS

Computed tomography (CT)-guided lung biopsies were conducted on 300 patients diagnosed with peripheral lung cancer. The patients were randomly assigned to two groups for C-ROSE using either rapid HE staining or DQ staining, and subsequently the two methods were compared and evaluated.

RESULTS

The concordance rate between C-ROSE and histopathological diagnosis was 96.7%. The median staining time for rapid HE staining was 160 s, while that for DQ staining was 120 s, representing a significant difference between the two groups (P<0.001). However, there were no significant differences observed in terms of total biopsy time, concordance rate with histopathology, cytology specimen peeling rate, and incidence of serious adverse reactions between the two groups (P>0.05).

CONCLUSIONS

Both staining methods comply with C-ROSE criteria in the biopsy setting of peripheral lung cancer. Rapid HE staining is more aligned with domestic clinical requirements and holds potential for further promotion and adoption in C-ROSE.

【中文题目：快速HE染色在周围型肺癌穿刺活检
快速现场细胞学评价中的应用】 【中文摘要：背景与目的 快速现场评价（rapid on-site evaluation, ROSE）是在活检术中同步实施的标本快速细胞学染色和评价技术。迪夫（Diff-Quik, DQ）染色是快速现场细胞学评价（cytological ROSE, C-ROSE）最常用的染色方法，但国内大部分病理医生不使用DQ染色进行现场细胞学判读，因此难以开展C-ROSE。本研究拟在周围型肺癌穿刺活检过程中分别使用快速苏木素-伊红（hematoxylin-eosin, HE）染色和DQ染色进行C-ROSE，评估快速HE染色在C-ROSE中的应用价值。方法 对300例术前拟诊周围型肺癌患者实施胸部计算机断层扫描（computed tomography, CT）引导肺活检，随机分成两组且分别使用快速HE染色和DQ染色，进行C-ROSE判读，并对两种染色方法进行比较和评价。结果 C-ROSE与组织病理诊断符合率为96.7%。快速HE染色的中位时间为160 s，而DQ染色为120 s，两组存在统计学差异（P<0.001）。但两组的肺活检时间、组织病理符合率、细胞学标本脱片率和严重不良反应发生率均无统计学差异（P>0.05）。结论 在周围型肺癌活检过程中，两种染色方法均能满足C-ROSE要求，快速HE染色可应用于C-ROSE，且更符合国内临床需求，具有潜在的推广价值。
】 【中文关键词：肺肿瘤；周围型肺癌；CT引导肺活检；快速现场细胞学评价；快速HE染色】.