A database of restriction maps to expand the utility of bacterial artificial chromosomes.
Journal
GigaByte (Hong Kong, China)
ISSN: 2709-4715
Titre abrégé: GigaByte
Pays: China
ID NLM: 101775049
Informations de publication
Date de publication:
2023
2023
Historique:
received:
03
05
2023
accepted:
18
09
2023
medline:
27
9
2023
pubmed:
27
9
2023
entrez:
27
9
2023
Statut:
epublish
Résumé
While Bacterial Artificial Chromosomes libraries were once a key resource for the genomic community, they have been obviated, for sequencing purposes, by long-read technologies. Such libraries may now serve as a valuable resource for manipulating and assembling large genomic constructs. To enhance accessibility and comparison, we have developed a BAC restriction map database. Using information from the National Center for Biotechnology Information's cloneDB FTP site, we constructed a database containing the restriction maps for both uniquely placed and insert-sequenced BACs from 11 libraries covering the recognition sequences of the available restriction enzymes. Along with the database, we generated a set of Python functions to reconstruct the database and more easily access the information within. This data is valuable for researchers simply using BACs, as well as those working with larger sections of the genome in terms of synthetic genes, large-scale editing, and mapping.
Identifiants
pubmed: 37753479
doi: 10.46471/gigabyte.93
pii: 93
pmc: PMC10518450
doi:
Types de publication
Journal Article
Langues
eng
Pagination
gigabyte93Subventions
Organisme : NHGRI NIH HHS
ID : R21 HG012281
Pays : United States
Organisme : NHGRI NIH HHS
ID : T32 HG002760
Pays : United States
Informations de copyright
© The Author(s) 2023.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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