(R)evolution of the Standard Addition Procedure for Immunoassays.
(bio-)sensor
calibration-free
immunoassay
label-free
reflectometric interference spectroscopy (RIfS)
standard addition
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
25 Aug 2023
25 Aug 2023
Historique:
received:
27
07
2023
revised:
18
08
2023
accepted:
24
08
2023
medline:
28
9
2023
pubmed:
27
9
2023
entrez:
27
9
2023
Statut:
epublish
Résumé
A new method to transfer the standard addition procedure for concentration determination to immunoassays with non-linear calibration curves was developed. The new method was successfully applied to simulated data and benchmarked against a state-of-the-art algorithm, showing a significantly improved performance with improvement factors between 2 and 192. The logit function was used to transform the immunoassay signal response of test samples spiked with known analyte concentrations. The relationship between logit(signal) and log-transformed estimated total analyte concentration is linear if the estimated total analyte concentration is correct. Finally, the new method was validated experimentally using different assays in varying, relevant complex matrices, such as serum, saliva, and milk. Different concentrations of testosterone and amitriptyline between 0.05 and 3.0 µg L
Identifiants
pubmed: 37754083
pii: bios13090849
doi: 10.3390/bios13090849
pmc: PMC10526245
pii:
doi:
Substances chimiques
Amitriptyline
1806D8D52K
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Bundesministerium für Wirtschaft und Energie
ID : 20949 N/2
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