Exploring the Impact of Organic Solvent Quality and Unusual Adduct Formation during LC-MS-Based Lipidomic Profiling.

MS/MS annotation adduct formation lipidomics lipids liquid chromatography mass spectrometry metabolomics method development misidentification solvent quality

Journal

Metabolites
ISSN: 2218-1989
Titre abrégé: Metabolites
Pays: Switzerland
ID NLM: 101578790

Informations de publication

Date de publication:
22 Aug 2023
Historique:
received: 03 08 2023
revised: 19 08 2023
accepted: 21 08 2023
medline: 27 9 2023
pubmed: 27 9 2023
entrez: 27 9 2023
Statut: epublish

Résumé

Liquid chromatography-mass spectrometry (LC-MS) is the key technique for analyzing complex lipids in biological samples. Various LC-MS modes are used for lipid separation, including different stationary phases, mobile-phase solvents, and modifiers. Quality control in lipidomics analysis is crucial to ensuring the generated data's reliability, reproducibility, and accuracy. While several quality control measures are commonly discussed, the impact of organic solvent quality during LC-MS analysis is often overlooked. Additionally, the annotation of complex lipids remains prone to biases, leading to potential misidentifications and incomplete characterization of lipid species. In this study, we investigate how LC-MS-grade isopropanol from different vendors may influence the quality of the mobile phase used in LC-MS-based untargeted lipidomic profiling of biological samples. Furthermore, we report the occurrence of an unusual, yet highly abundant, ethylamine adduct [M+46.0651]

Identifiants

pubmed: 37755246
pii: metabo13090966
doi: 10.3390/metabo13090966
pmc: PMC10536874
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Ministry of Health
ID : NU20-01-00186
Organisme : Ministry of Health
ID : NU22-02-00161
Organisme : Czech Science Foundation
ID : 21-00477S
Organisme : Ministry of Education Youth and Sports
ID : LTAUSA19124
Organisme : Ministry of Education Youth and Sports
ID : LX22NPO5104
Organisme : Czech Academy of Sciences
ID : LQ200111901

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Auteurs

Tomas Cajka (T)

Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic.

Jiri Hricko (J)

Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic.

Lucie Rudl Kulhava (L)

Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic.

Michaela Paucova (M)

Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic.

Michaela Novakova (M)

Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic.

Oliver Fiehn (O)

West Coast Metabolomics Center, University of California Davis, 451 Health Sciences Drive, Davis, CA 95616, USA.

Ondrej Kuda (O)

Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 14200 Prague, Czech Republic.

Classifications MeSH