Creation of a decellularized vaginal matrix from healthy human vaginal tissue for potential vagina reconstruction - experimental studies.
Journal
International journal of surgery (London, England)
ISSN: 1743-9159
Titre abrégé: Int J Surg
Pays: United States
ID NLM: 101228232
Informations de publication
Date de publication:
27 Sep 2023
27 Sep 2023
Historique:
received:
26
06
2023
accepted:
21
08
2023
medline:
27
9
2023
pubmed:
27
9
2023
entrez:
27
9
2023
Statut:
aheadofprint
Résumé
When a disorder causes absence of a healthy, full-size vagina, various neovaginal creation methods are available. Sometimes dilation or stretching of the vaginal cavity is sufficient, but intestinal or dermal flap tissue is generally required. However, different inherent tissue properties cause complications. Therefore, a lost body part should be replaced with a similar material. The use of organ-specific acellular vaginal tissue carries great potential, as its similar architecture and matrix composition make it suitable for vaginal regeneration. We developed an optimized protocol for decellularization of healthy, human vaginal tissue. Resected colpectomy tissue from 12 healthy transgender patients was used. Successful decellularization was confirmed by applying acellular criteria from in vivo remodeling-reports. Suitability as tissue-mimicking scaffold for vaginal reconstruction was determined by visible structural features, biocompatibility during stretching and presence of visible collagen, elastin, laminin and fibronectin. Histological examination confirmed the preservation of structural features and minimal cellular residue was seen during fluorescence microscopy, DNA and RNA quantification and fragment-length examination. Biomechanical testing showed decreased peak load (55%, P<0.05), strain at rupture (23%, P<0.01) and ultimate tensile stress (55%, P<0.05) after decellularization, while the elastic modulus (68%) did not decrease significantly. Fluorescence microscopy revealed preserved Fibronectin-I/II/III and Laminin-I/II, while Collagen-I and Ficolin-2B were decreased but mostly retained. The absence of cellular residue, moderately altered biomechanical extracellular matrix (ECM) properties and mostly preserved structural proteins, appear to make our decellularized human vaginal matrix a suitable tissue-mimicking scaffold for vagina transplantation when tissue survival through vascularization and innervation are accomplished in the future.
Sections du résumé
BACKGROUND
BACKGROUND
When a disorder causes absence of a healthy, full-size vagina, various neovaginal creation methods are available. Sometimes dilation or stretching of the vaginal cavity is sufficient, but intestinal or dermal flap tissue is generally required. However, different inherent tissue properties cause complications. Therefore, a lost body part should be replaced with a similar material. The use of organ-specific acellular vaginal tissue carries great potential, as its similar architecture and matrix composition make it suitable for vaginal regeneration.
METHODS
METHODS
We developed an optimized protocol for decellularization of healthy, human vaginal tissue. Resected colpectomy tissue from 12 healthy transgender patients was used. Successful decellularization was confirmed by applying acellular criteria from in vivo remodeling-reports. Suitability as tissue-mimicking scaffold for vaginal reconstruction was determined by visible structural features, biocompatibility during stretching and presence of visible collagen, elastin, laminin and fibronectin.
RESULTS
RESULTS
Histological examination confirmed the preservation of structural features and minimal cellular residue was seen during fluorescence microscopy, DNA and RNA quantification and fragment-length examination. Biomechanical testing showed decreased peak load (55%, P<0.05), strain at rupture (23%, P<0.01) and ultimate tensile stress (55%, P<0.05) after decellularization, while the elastic modulus (68%) did not decrease significantly. Fluorescence microscopy revealed preserved Fibronectin-I/II/III and Laminin-I/II, while Collagen-I and Ficolin-2B were decreased but mostly retained.
CONCLUSIONS
CONCLUSIONS
The absence of cellular residue, moderately altered biomechanical extracellular matrix (ECM) properties and mostly preserved structural proteins, appear to make our decellularized human vaginal matrix a suitable tissue-mimicking scaffold for vagina transplantation when tissue survival through vascularization and innervation are accomplished in the future.
Identifiants
pubmed: 37755377
doi: 10.1097/JS9.0000000000000727
pii: 01279778-990000000-00680
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc.