Gene regulation for inflammation and inflammation resolution differs between umbilical arterial and venous endothelial cells.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 09 2023
27 09 2023
Historique:
received:
27
05
2023
accepted:
20
09
2023
medline:
3
11
2023
pubmed:
28
9
2023
entrez:
27
9
2023
Statut:
epublish
Résumé
Systemic inflammation affects the whole vasculature, yet whether arterial and venous endothelial cells differ in their abilities to mediate inflammation and to return to homeostasis after an inflammatory stimulus has not been addressed thoroughly. We assessed gene-expression profiles in isolated endothelial cells from human umbilical arteries (HUAEC) or veins (HUVEC) under basal conditions, after TNF-α stimulation and various time points after TNF-α removal to allow reinstatement of homeostasis. TNF-α regulates the expression of different sets of transcripts that are significantly changed only in HUAEC, only in HUVEC or changed in both. We identified three types of gene regulation, i.e. genes that were significantly regulated after 24 h of TNF-α stimulation but no longer when TNF-α was removed (homeostatic regulation), genes that maintained significantly regulated after TNF-α removal (not homeostatic regulation) and genes that were only significantly regulated when TNF-α was removed (post-regulation). HUAEC and HUVEC quantitatively differed in these types of gene regulation, with relatively more genes being post-regulated in HUAEC. In conclusion our data demonstrate that HUAEC and HUVEC respond intrinsically different to an inflammatory insult. Whether this holds true for all endothelial cells and its relevance for inflammatory insults in different organs during systemic inflammation warrants further studies.
Identifiants
pubmed: 37758738
doi: 10.1038/s41598-023-43142-6
pii: 10.1038/s41598-023-43142-6
pmc: PMC10533526
doi:
Substances chimiques
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16159Informations de copyright
© 2023. Springer Nature Limited.
Références
Cardiovasc Res. 2001 Feb 16;49(3):507-21
pubmed: 11166264
Circ Res. 2018 Feb 2;122(3):417-432
pubmed: 29208678
Am J Pathol. 2017 Jun;187(6):1211-1221
pubmed: 28527709
Nature. 2021 Jan;589(7842):437-441
pubmed: 33299176
Stem Cell Res Ther. 2019 Jul 29;10(1):216
pubmed: 31358056
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):8713-8720
pubmed: 31696457
Nucleic Acids Res. 2005 Nov 10;33(20):e175
pubmed: 16284200
Cytokine Growth Factor Rev. 2004 Aug;15(4):237-54
pubmed: 15207815
Crit Care Med. 2003 Apr;31(4 Suppl):S221-30
pubmed: 12682444
Dev Cell. 2004 Apr;6(4):479-82
pubmed: 15068788
Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10623-8
pubmed: 12963823
PLoS One. 2018 Oct 18;13(10):e0206239
pubmed: 30335862
Nat Rev Immunol. 2003 Sep;3(9):745-56
pubmed: 12949498
Physiol Genomics. 2007 Jan 17;28(2):179-92
pubmed: 17234577
Blood. 2007 Jun 1;109(11):4777-85
pubmed: 17289814
Chest. 2000 Aug;118(2):503-8
pubmed: 10936147
Microvasc Res. 1998 Jan;55(1):65-76
pubmed: 9473410
Vascular. 2022 Feb;30(1):151-161
pubmed: 33530884
Front Endocrinol (Lausanne). 2018 Aug 03;9:402
pubmed: 30123182
Semin Immunopathol. 2019 Nov;41(6):675-679
pubmed: 31720751
J Physiol. 2018 Sep;596(17):3929-3949
pubmed: 29885204
Eur J Clin Invest. 2009 Apr;39(4):320-7
pubmed: 19292888
Arterioscler Thromb Vasc Biol. 1997 Jul;17(7):1193-202
pubmed: 9261246
Nat Rev Immunol. 2013 Jan;13(1):59-66
pubmed: 23197111
Sci Rep. 2019 Feb 4;9(1):1152
pubmed: 30718683
Endothelium. 2001;8(1):25-40
pubmed: 11409849
Circ Res. 2020 Mar 27;126(7):875-888
pubmed: 32065070
Nat Commun. 2018 May 8;9(1):1828
pubmed: 29739946
Blood. 2013 Dec 5;122(24):3982-92
pubmed: 24108462