Exploring Immunohistochemistry in Fish: Assessment of Antibody Reactivity by Western Immunoblotting.
GFAP
S-100
antibody validation
aquaculture
cytokeratin
desmin
fish pathology
immunohistochemistry
vimentin
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
15 Sep 2023
15 Sep 2023
Historique:
received:
04
08
2023
revised:
04
09
2023
accepted:
14
09
2023
medline:
28
9
2023
pubmed:
28
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
In recent years, research on fish has seen remarkable advancements, especially in aquaculture, ornamental fish industry, and biomedical studies. Immunohistochemistry has become crucial in fish research, aiding in physiological and pathological investigations. However, the use of antibodies originally developed for mammals has raised concerns about their cross-reactivity and specificity in fish. This study systematically evaluated the reactivity of commonly used antibodies for diagnostic purposes, especially in fish pathology, including pan-cytokeratin, vimentin, S-100, glial fibrillary acidic protein, and desmin in the tissue of
Identifiants
pubmed: 37760333
pii: ani13182934
doi: 10.3390/ani13182934
pmc: PMC10525475
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : This research was supported by the University of Sassari (Fondo di Ateneo per la Ricerca 2019-2020) and by the University of Milan (M.F.A., personal funds).
ID : x
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