Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Modulate Apoptosis, TNF Alpha and Interferon Gamma Response Gene mRNA Expression in T Lymphocytes.

Ingenuity Pathway Analysis T lymphocytes gene set enrichment analysis mesenchymal stem cells next-generation sequencing small extracellular vesicles

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
05 Sep 2023
Historique:
received: 12 07 2023
revised: 29 08 2023
accepted: 30 08 2023
medline: 29 9 2023
pubmed: 28 9 2023
entrez: 28 9 2023
Statut: epublish

Résumé

Recent studies have highlighted the therapeutic potential of small extracellular bodies derived from mesenchymal stem cells (MSC-sEVs) for various diseases, notably through their ability to alter T-cell differentiation and function. The current study aimed to explore immunomodulatory pathway alterations within T cells through mRNA sequencing of activated T cells cocultured with bone marrow-derived MSC-sEVs. mRNA profiling of activated human T cells cocultured with MSC-sEVs or vehicle control was performed using the QIAGEN Illumina sequencing platform. Pathway networks and biological functions of the differentially expressed genes were analyzed using Ingenuity pathway analysis (IPA)

Identifiants

pubmed: 37761990
pii: ijms241813689
doi: 10.3390/ijms241813689
pmc: PMC10530670
pii:
doi:

Substances chimiques

Tumor Necrosis Factor-alpha 0
Interferon-gamma 82115-62-6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Ministry of Science & Technology, Israel
ID : 0000-0000
Organisme : Department of Science and Technology, Government of India
ID : 0000-0000

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Auteurs

Andrea Fracchia (A)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.
Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Drirh Khare (D)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.
Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Samar Da'na (S)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.

Reuven Or (R)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.
Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Amnon Buxboim (A)

Department of Cell and Developmental Biology, Hebrew University of Jerusalem, Jerusalem 9190401, Israel.

Boaz Nachmias (B)

Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.
Department of Hematology, Hadassah Medical Center, Jerusalem 9112001, Israel.

Claudine Barkatz (C)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.

Regina Golan-Gerstl (R)

Department of Pediatrics, Hadassah-Hebrew University Medical Center, Jerusalem 9112001, Israel.

Swasti Tiwari (S)

Department of Molecular Medicine & Biotechnology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, India.

Polina Stepensky (P)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.
Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Yuval Nevo (Y)

Info-CORE, Bioinformatics Unit of the I-CORE at the Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Hadar Benyamini (H)

Info-CORE, Bioinformatics Unit of the I-CORE at the Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Sharona Elgavish (S)

Info-CORE, Bioinformatics Unit of the I-CORE at the Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

Osnat Almogi-Hazan (O)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.

Batia Avni (B)

Department of Bone Marrow Transplantation & Cancer Immunotherapy, Hadassah Medical Center, Jerusalem 9112001, Israel.
Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem 9112001, Israel.

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Classifications MeSH