Method Based on Ultrafiltration to Obtain a Plasma Rich in Platelet and Plasma Growth Factors.
biomolecules
growth factors
hydrogel
platelet-rich plasma
platelets
water filtration
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
13 Sep 2023
13 Sep 2023
Historique:
received:
04
08
2023
revised:
30
08
2023
accepted:
11
09
2023
medline:
28
9
2023
pubmed:
28
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
Platelet-Rich Plasma (PRP) is an autologous biological product which, due to its regenerative capacity, is currently used in different fields of medicine. This biological treatment has proven to be effective in numerous research studies due to its high content of growth factors released by platelets. However, the current systems used to obtain PRP do not enrich the growth factors and cytokines outside platelets. Considering this, the present work aims to develop a new technique by which all the biomolecules present in plasma are enriched. Thus, a new method based on ultrafiltration has been developed for the obtaining of the novel PRP. By this method, ultrafiltration of the plasma water is carried out using a 3KDa filtering unit. The results showed that the technique was able to concentrate extraplatelet factors, such as IGF-1 and HGF, in contrast with conventional plasmas. Thus, the cultured cells responded with increased viability to this new PRP. These results could provide a new approach to the treatment of injuries requiring regenerative medicine, potentially improving the outcomes of the conventional PRPs.
Identifiants
pubmed: 37762883
pii: jcm12185941
doi: 10.3390/jcm12185941
pmc: PMC10532222
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministerio de Ciencia y Educación de España
ID : PID2020-120313GB-I00/AIE/10.13039/501100011033
Organisme : Gobierno Vasco Dpto. de Educación
ID : IT1633-22
Organisme : Ayudas para la formación de personal investigador en colaboración con instituciones y empresas
ID : PIFIND19/03
Organisme : Spanish Ministerio de Economía y Competitividad
ID : PTQ- 3122019-010386
Références
J Clin Med. 2020 Jul 26;9(8):
pubmed: 32722531
Mil Med. 2014 Jul;179(7):799-805
pubmed: 25003868
Nature. 2017 Apr 6;544(7648):105-109
pubmed: 28329764
Sports Med Arthrosc Rev. 2013 Dec;21(4):180-5
pubmed: 24212364
Dent Implantol Update. 2000 Mar;11(3):17-21
pubmed: 11992937
PLoS One. 2013 Jun 28;8(6):e67303
pubmed: 23840657
Am J Sports Med. 2007 Feb;35(2):245-51
pubmed: 17099241
PLoS One. 2018 Jan 18;13(1):e0190827
pubmed: 29346441
Int J Mol Sci. 2023 Mar 10;24(6):
pubmed: 36982439
Int J Oral Maxillofac Implants. 2003 Jan-Feb;18(1):93-103
pubmed: 12608674
J Oral Maxillofac Surg. 2004 Apr;62(4):489-96
pubmed: 15085519
J Exp Orthop. 2019 Feb 2;6(1):4
pubmed: 30712144
Int J Mol Sci. 2021 Aug 16;22(16):
pubmed: 34445472
Int J Mol Sci. 2021 Jan 15;22(2):
pubmed: 33467646
Int J Mol Sci. 2022 Mar 05;23(5):
pubmed: 35270001
IEEE Trans Biomed Eng. 2016 Aug;63(8):1642-52
pubmed: 26126268
J Appl Physiol (1985). 2006 Jul;101(1):183-8
pubmed: 16782835
Crit Care. 2000;4(1):6-14
pubmed: 11094491
Biomed Microdevices. 2012 Dec;14(6):1095-102
pubmed: 22811077
Life Sci. 1991;49(26):1997-2003
pubmed: 1749310
J Biol Regul Homeost Agents. 2012 Apr-Jun;26(2 Suppl 1):3S-22S
pubmed: 23648195
J Orthop Surg Res. 2019 Feb 6;14(1):39
pubmed: 30728046
Expert Opin Biol Ther. 2020 Dec;20(12):1447-1460
pubmed: 32692595
Lab Chip. 2020 Mar 3;20(5):949-957
pubmed: 31989123
Wounds. 2011 Jun;23(6):171-82
pubmed: 25881364
J Cutan Aesthet Surg. 2014 Oct-Dec;7(4):189-97
pubmed: 25722595
J Cell Physiol. 2010 Nov;225(3):757-66
pubmed: 20568106