A Whole-Genome Sequencing-Based Approach for the Characterization of
Klebsiella pneumoniae
carbapenemases
whole-genome sequencing
β-lactam/β-lactamases inhibitors
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
20 Sep 2023
20 Sep 2023
Historique:
received:
07
08
2023
revised:
16
09
2023
accepted:
18
09
2023
medline:
28
9
2023
pubmed:
28
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
Whole-genome sequencing (WGS) provides important information for the characterization, surveillance, and monitoring of antimicrobial resistance (AMR) determinants, particularly in cases of multi- and extensively drug-resistant microorganisms. We reported the results of a WGS analysis carried out on carbapenemases-producing Clinical, phenotypic, and genotypic data were collected for the AMR surveillance screening program of the University Hospital of Sassari (Italy) during 2020-2021. Genomic DNA was sequenced using the Illumina Nova Seq 6000 platform. Final assemblies were manually curated and carefully verified for the detection of antimicrobial resistance genes, porin mutations, and virulence factors. A phylogenetic analysis was performed using the maximum likelihood method. All 17 strains analyzed belonged to ST512, and most of them carried the WGS characterization revealed the presence of several antibiotic resistance determinants and porin mutations in highly resistant
Sections du résumé
BACKGROUND
BACKGROUND
Whole-genome sequencing (WGS) provides important information for the characterization, surveillance, and monitoring of antimicrobial resistance (AMR) determinants, particularly in cases of multi- and extensively drug-resistant microorganisms. We reported the results of a WGS analysis carried out on carbapenemases-producing
METHODS
METHODS
Clinical, phenotypic, and genotypic data were collected for the AMR surveillance screening program of the University Hospital of Sassari (Italy) during 2020-2021. Genomic DNA was sequenced using the Illumina Nova Seq 6000 platform. Final assemblies were manually curated and carefully verified for the detection of antimicrobial resistance genes, porin mutations, and virulence factors. A phylogenetic analysis was performed using the maximum likelihood method.
RESULTS
RESULTS
All 17 strains analyzed belonged to ST512, and most of them carried the
CONCLUSION
CONCLUSIONS
WGS characterization revealed the presence of several antibiotic resistance determinants and porin mutations in highly resistant
Identifiants
pubmed: 37764198
pii: microorganisms11092354
doi: 10.3390/microorganisms11092354
pmc: PMC10535212
pii:
doi:
Types de publication
Journal Article
Langues
eng
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