Human Pegivirus-1 Detection and Genotyping in Brazilian Patients with Fulminant Hepatitis.
HPgV-1
fulminant hepatitis
genotyping
human pegvirus-1
qPCR
Journal
Pathogens (Basel, Switzerland)
ISSN: 2076-0817
Titre abrégé: Pathogens
Pays: Switzerland
ID NLM: 101596317
Informations de publication
Date de publication:
01 Sep 2023
01 Sep 2023
Historique:
received:
24
04
2023
revised:
20
07
2023
accepted:
11
08
2023
medline:
28
9
2023
pubmed:
28
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
Fulminant hepatitis is a severe clinical disease characterized by a marked decline in liver function and encephalopathy. In a previous survey, using metagenomics in a group of 27 patients with this clinical condition, we observed an expressive quantity of reads of the Human pegivirus-1 (HPgV-1). Therefore, the objective of this study was to evaluate the frequency, molecular features, and HPgV-1 circulating genotypes in patients with fulminant hepatitis. After testing the collected plasma samples, we discovered twelve samples (44.4%) that were positive for HPgV-1 RNA (using both real-time and nested PCR). The positive samples presented a mean cycle threshold (Ct) of 28.5 (±7.3). Genotyping assignments revealed that all HPgV-1 positive samples belonged to the HPgV-1 genotype 2 (both subgenotypes 2A and 2B were identified). Although HPgV-1 is considered a commensal virus, little is known regarding its prevalence and genotypes in cases of fulminant hepatitis. More research is needed to understand whether HPgV-1 can be implicated in clinical disorders and infectious diseases.
Identifiants
pubmed: 37764930
pii: pathogens12091122
doi: 10.3390/pathogens12091122
pmc: PMC10536510
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : São Paulo Research Foundation
ID : 2018/15826-5
Organisme : São Paulo Research Foundation
ID : 2017/23205-8
Organisme : São Paulo Research Foundation
ID : 2019/08528-0
Organisme : São Paulo Research Foundation
ID : 2021/11944-6
Organisme : São Paulo Research Foundation
ID : 2022/00910-6
Organisme : São Paulo Research Foundation
ID : 2022/10278-5
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