Construing recombinant ZFP160 from Aspergillus terreus as pterin deaminase enzyme.
Aspergillus terreus
Molecular Cloning
amidohydrolase
pterin deaminase
zfp160
Journal
Biotechnology and applied biochemistry
ISSN: 1470-8744
Titre abrégé: Biotechnol Appl Biochem
Pays: United States
ID NLM: 8609465
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
17
12
2022
accepted:
09
09
2023
pubmed:
28
9
2023
medline:
28
9
2023
entrez:
28
9
2023
Statut:
ppublish
Résumé
Pterin deaminase stands as a metalloenzyme and exhibits both antitumor and anticancer activities. Therefore, this study aimed to explore the molecular function of zinc finger protein-160 (zfp160) from Aspergillus terreus with its enzyme mechanism in detail. Subsequently, preliminary molecular docking studies on zfp160 from A. terreus were done. Next, the cloning and expression of zfp160 protein were carried out. Following, protein expression was induced and purified through nickel NTA column with imidazole gradient elution. Through the Mascot search engine tool, the expressed protein of MALDI-TOF was confirmed by 32 kDa bands of SDS-PAGE. Furthermore, its enzymatic characterization and biochemical categorization were also explored. The optimum conditions for enzyme were determined to be pH 8, temperature 35°C, km 50 μm with folic acid as substrate, and V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2150-2162Informations de copyright
© 2023 International Union of Biochemistry and Molecular Biology, Inc.
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