Bst polymerase - a humble relative of Taq polymerase.
Bst polymerase
DNA polymerase
Directed evolution
Fidelity
Fusion proteins
Processivity
Site-directed mutagenesis
Strand displacement
Journal
Computational and structural biotechnology journal
ISSN: 2001-0370
Titre abrégé: Comput Struct Biotechnol J
Pays: Netherlands
ID NLM: 101585369
Informations de publication
Date de publication:
2023
2023
Historique:
received:
14
05
2023
revised:
31
08
2023
accepted:
10
09
2023
medline:
28
9
2023
pubmed:
28
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair. Modern biotechnology and molecular diagnostics rely heavily on DNA polymerases in analyzing nucleic acids. Among a variety of discovered DNA polymerases, Bst polymerase, a large fragment of DNA polymerase I from Geobacillus stearothermophilus, is one of the most commonly used but is not as well studied as Taq polymerase. The ability of Bst polymerase to displace an upstream DNA strand during synthesis, coupled with its moderate thermal stability, has provided the basis for several isothermal DNA amplification methods, including LAMP, WGA, RCA, and many others. Bst polymerase is one of the key components defining the robustness and analytical characteristics of diagnostic test systems based on isothermal amplification. Here, we present an overview of the biochemical and structural features of Bst polymerase and provide information on its mutated analogs.
Identifiants
pubmed: 37767105
doi: 10.1016/j.csbj.2023.09.008
pii: S2001-0370(23)00321-5
pmc: PMC10520511
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
4519-4535Informations de copyright
© 2023 The Authors.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
Références
Genet Anal. 1996 Mar;12(5-6):185-95
pubmed: 8740835
Proc Natl Acad Sci U S A. 2005 May 3;102(18):6407-12
pubmed: 15845765
Gene. 1995 Sep 22;163(1):65-8
pubmed: 7557480
ACS Synth Biol. 2022 Apr 15;11(4):1488-1496
pubmed: 35320674
Nucleic Acids Res. 2000 Jun 15;28(12):E63
pubmed: 10871386
Nucleic Acids Res. 2016 Sep 30;44(17):8229-40
pubmed: 27325747
J Bacteriol. 1981 Jan;145(1):21-6
pubmed: 7462144
Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14598-603
pubmed: 17785419
Structure. 1997 Jan 15;5(1):95-108
pubmed: 9016716
Nature. 2004 Sep 9;431(7005):217-21
pubmed: 15322558
PLoS One. 2009;4(3):e4949
pubmed: 19305495
FEMS Microbiol Lett. 2014 Aug;357(2):144-50
pubmed: 24965874
Biochemistry. 1988 Aug 9;27(16):6008-13
pubmed: 2847780
PLoS One. 2018 Jun 20;13(6):e0199559
pubmed: 29924849
Nucleic Acids Res. 2023 Feb 28;51(4):1750-1765
pubmed: 36744436
Nucleic Acids Res. 1998 Oct 15;26(20):4652-6
pubmed: 9753733
Biochemistry. 2004 Oct 26;43(42):13459-66
pubmed: 15491153
J Biol Chem. 2004 Nov 26;279(48):50280-5
pubmed: 15385534
Biochemistry. 2009 Sep 1;48(34):8271-8
pubmed: 19642651
Sci Sin B. 1987 May;30(5):503-6
pubmed: 3672099
Biochemistry. 2012 Mar 13;51(10):2032-43
pubmed: 22320201
J Biol Chem. 1999 Jan 29;274(5):3067-75
pubmed: 9915846
Protein Sci. 1996 Sep;5(9):1785-92
pubmed: 8880902
Anal Bioanal Chem. 2022 Sep;414(21):6309-6326
pubmed: 35657389
Biochemistry. 2018 Aug 7;57(31):4607-4619
pubmed: 29629759
Biotechniques. 2001 Apr;30(4):852-6, 858, 860 passim
pubmed: 11314268
J Biol Chem. 1975 Nov 25;250(22):8647-56
pubmed: 1102540
J Biotechnol. 2009 Dec;144(4):245-52
pubmed: 19770007
Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19701-6
pubmed: 17179038
J Biol Chem. 2016 Sep 30;291(40):20869-20875
pubmed: 27462081
Chemistry. 2008;14(7):2125-32
pubmed: 18196510
Virology. 2007 Sep 30;366(2):361-76
pubmed: 17532359
Nat Struct Biol. 1998 Nov;5(11):959-64
pubmed: 9808040
Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):980-985
pubmed: 29339523
AIMS Biophys. 2018;5(2):125-143
pubmed: 29888334
J Biol Chem. 1969 Jun 10;244(11):3045-52
pubmed: 4890764
Chin J Biotechnol. 1992;8(1):29-32
pubmed: 1457719
ACS Synth Biol. 2021 Jun 18;10(6):1429-1437
pubmed: 34029459
Proc Natl Acad Sci U S A. 1995 Jul 3;92(14):6339-43
pubmed: 7603992
J Biol Chem. 2005 Feb 4;280(5):3764-70
pubmed: 15548515
Analyst. 2020 Jan 21;145(2):440-444
pubmed: 31793929
J Biol Chem. 1989 Apr 15;264(11):6427-37
pubmed: 2649500
J Biol Chem. 1990 Aug 15;265(23):13878-87
pubmed: 2199444
Biotechniques. 1991 Jul;11(1):76-8, 80, 82-7
pubmed: 1954022
J Biol Chem. 1990 Aug 25;265(24):14579-91
pubmed: 2201688
Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9491-6
pubmed: 10449720
Nature. 1998 Jan 15;391(6664):304-7
pubmed: 9440698
J Biol Chem. 2001 Dec 14;276(50):46759-64
pubmed: 11602576
Proc Natl Acad Sci U S A. 1970 Oct;67(2):1034-41
pubmed: 4331715
Biotechniques. 1991 Oct;11(4):464, 466
pubmed: 1793578
Nucleic Acids Res. 2009 Jan;37(1):193-203
pubmed: 19033368
J Biol Chem. 2012 Aug 17;287(34):28215-26
pubmed: 22648417
J Biol Chem. 1969 Jun 10;244(11):3038-44
pubmed: 4977234
BMC Mol Cell Biol. 2019 Aug 9;20(1):31
pubmed: 31399021
Front Bioeng Biotechnol. 2021 Jan 14;8:553474
pubmed: 33520948
ACS Chem Biol. 2016 Nov 18;11(11):3165-3171
pubmed: 27668519
Bioorg Khim. 2012 Mar-Apr;38(2):199-205
pubmed: 22792723
J Bacteriol. 1976 Sep;127(3):1550-7
pubmed: 8432
Chembiochem. 2016 Oct 4;17(19):1804-1808
pubmed: 27383648
Biochemistry. 1991 Aug 6;30(31):7661-6
pubmed: 1714296
Nucleic Acids Res. 2017 Sep 19;45(16):9595-9610
pubmed: 28934494
J Am Chem Soc. 2003 Jan 29;125(4):856-7
pubmed: 12537469
Chem Sci. 2018 Mar 5;9(14):3544-3554
pubmed: 29780486
Nucleic Acids Res. 2020 Apr 17;48(7):e42
pubmed: 32103255
Biochemistry. 2023 Jan 17;62(2):410-418
pubmed: 34762799
Biol Chem. 2007 Apr;388(4):367-72
pubmed: 17391057
Nat Commun. 2018 Nov 29;9(1):5067
pubmed: 30498223
J Biomol Struct Dyn. 2019 Aug;37(13):3314-3321
pubmed: 30146945
J Am Chem Soc. 2015 Nov 4;137(43):13804-6
pubmed: 26474356
Angew Chem Int Ed Engl. 2016 Dec 19;55(51):15856-15859
pubmed: 27879047
Biochemistry. 2002 Jan 22;41(3):713-22
pubmed: 11790092
Biokhimiia. 1981 Apr;46(4):681-90
pubmed: 7284483
Biochim Biophys Acta. 1977 Mar 2;475(1):32-41
pubmed: 849445
Biotechniques. 1998 Mar;24(3):392-6
pubmed: 9526646
J Biol Chem. 1987 Nov 25;262(33):16212-23
pubmed: 3316214
J Biochem. 1993 Mar;113(3):401-10
pubmed: 8486614
PLoS One. 2012;7(3):e33482
pubmed: 22432028
J Bacteriol. 1992 Jul;174(13):4350-5
pubmed: 1320608
Nucleic Acids Res. 1989 Oct 25;17(20):8387-8
pubmed: 2478963
Nucleic Acids Res. 2017 Dec 1;45(21):12425-12440
pubmed: 29040737
J Mol Biol. 2003 Jan 17;325(3):443-59
pubmed: 12498795
Biochemistry. 2009 Jun 2;48(21):4633-41
pubmed: 19348507
Biochimie. 2012 Sep;94(9):1968-73
pubmed: 22652043
Biomed Res Int. 2016;2016:2906484
pubmed: 27981047
Nucleic Acids Res. 2012 Jul;40(13):6174-86
pubmed: 22434889
Biochemistry. 2003 Mar 4;42(8):2373-85
pubmed: 12600204
J Mol Biol. 2001 Sep 14;312(2):323-33
pubmed: 11554789
DNA Seq. 1991;1(3):173-80
pubmed: 1773056
DNA Repair (Amst). 2020 May;89:102826
pubmed: 32113909
Appl Microbiol Biotechnol. 2009 Aug;84(1):105-17
pubmed: 19365630
Anal Biochem. 2009 Aug 15;391(2):85-90
pubmed: 19442643
DNA Seq. 1993;4(2):79-85
pubmed: 8173079
Bioorg Med Chem Lett. 2016 Jan 15;26(2):288-291
pubmed: 26707394
ACS Synth Biol. 2020 Nov 20;9(11):2936-2942
pubmed: 32966745
Proc Natl Acad Sci U S A. 1995 May 9;92(10):4641-5
pubmed: 7753856
BMC Genomics. 2006 Dec 12;7:312
pubmed: 17156491
Biochemistry. 1996 Jun 4;35(22):7256-66
pubmed: 8679555
Mitochondrion. 2013 Nov;13(6):592-601
pubmed: 23993955
J Biol Chem. 1978 Jan 25;253(2):413-23
pubmed: 338607
J Biol Chem. 1984 Aug 25;259(16):10386-92
pubmed: 6088498
Biosci Biotechnol Biochem. 2010;74(2):440-2
pubmed: 20139597
PCR Methods Appl. 1993 May;2(4):275-87
pubmed: 8324500
Nucleic Acids Res. 2015 Apr 30;43(8):4179-90
pubmed: 25813050
Nucleic Acids Res. 2018 Jun 20;46(11):5753-5763
pubmed: 29750267
J Biol Chem. 2007 Apr 6;282(14):10594-604
pubmed: 17259182
Nucleic Acids Res. 2004 Feb 18;32(3):1197-207
pubmed: 14973201
Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3895-900
pubmed: 12649320
Extremophiles. 2004 Jun;8(3):243-51
pubmed: 15197605
Cold Spring Harb Symp Quant Biol. 1968;33:1-9
pubmed: 4306813
Biochim Biophys Acta. 1996 Jun 7;1307(2):178-86
pubmed: 8679703