Middle meatus microbiome in patients with eosinophilic chronic rhinosinusitis in a Japanese population.

ALOX15 Fusobacterium LPS Sinusitis microbiota

Journal

The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002

Informations de publication

Date de publication:
Dec 2023
Historique:
received: 03 10 2022
revised: 21 06 2023
accepted: 30 06 2023
pubmed: 28 9 2023
medline: 28 9 2023
entrez: 28 9 2023
Statut: ppublish

Résumé

Chronic rhinosinusitis (CRS) is a common chronic inflammatory disease and is subdivided into eosinophilic and noneosinophilic forms. There are few reports investigating the nasal microbiome and its pathological functions in patients with CRS. We sought to analyze factors contributing to variations of the nasal microbiome in CRS, and on the basis of these factors, to elucidate whether the bacterial metabolites were related to the pathogenesis. Nasal swabs were collected, and the V3 to V4 variable region of the 16S ribosomal RNA gene was amplified and sequenced. Factors contributing to variations of the nasal microbiome in patients with CRS were compared. The most influential factor was whether CRS was eosinophilic, and we compared α- and β-diversity, bacterial species, and predictive bacterial functions between the 2 patient groups. In addition, the metabolites of the key bacteria were extracted, and we evaluated the predicted bacterial functions in airway epithelial cells. In total, 110 patients with CRS and 33 control subjects were enrolled. On the basis of the factors of variation, it was found that patients with eosinophilic CRS (n = 65) had different microbiomes with weighted UniFrac β-diversity and lower α-diversity compared with those with noneosinophilic CRS (n = 45). A higher abundance of Fusobacterium nucleatum and an increased LPS pathway were observed in patients with noneosinophilic CRS compared with those with eosinophilic CRS. In airway epithelial cells, LPS derived from F nucleatum suppressed the expression levels of ALOX15 induced by T The differences in the nasal microbiome may play a key role in the pathophysiology of CRS.

Sections du résumé

BACKGROUND BACKGROUND
Chronic rhinosinusitis (CRS) is a common chronic inflammatory disease and is subdivided into eosinophilic and noneosinophilic forms. There are few reports investigating the nasal microbiome and its pathological functions in patients with CRS.
OBJECTIVE OBJECTIVE
We sought to analyze factors contributing to variations of the nasal microbiome in CRS, and on the basis of these factors, to elucidate whether the bacterial metabolites were related to the pathogenesis.
METHODS METHODS
Nasal swabs were collected, and the V3 to V4 variable region of the 16S ribosomal RNA gene was amplified and sequenced. Factors contributing to variations of the nasal microbiome in patients with CRS were compared. The most influential factor was whether CRS was eosinophilic, and we compared α- and β-diversity, bacterial species, and predictive bacterial functions between the 2 patient groups. In addition, the metabolites of the key bacteria were extracted, and we evaluated the predicted bacterial functions in airway epithelial cells.
RESULTS RESULTS
In total, 110 patients with CRS and 33 control subjects were enrolled. On the basis of the factors of variation, it was found that patients with eosinophilic CRS (n = 65) had different microbiomes with weighted UniFrac β-diversity and lower α-diversity compared with those with noneosinophilic CRS (n = 45). A higher abundance of Fusobacterium nucleatum and an increased LPS pathway were observed in patients with noneosinophilic CRS compared with those with eosinophilic CRS. In airway epithelial cells, LPS derived from F nucleatum suppressed the expression levels of ALOX15 induced by T
CONCLUSIONS CONCLUSIONS
The differences in the nasal microbiome may play a key role in the pathophysiology of CRS.

Identifiants

DOI: 10.1016/j.jaci.2023.06.029 PMID: 37768238
pubmed: 37768238
pii: S0091-6749(23)00977-6
doi: 10.1016/j.jaci.2023.06.029
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1669-1676.e3

Informations de copyright

Copyright © 2023 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

Auteurs

Masanori Kidoguchi (M)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui; Department of Medical Genetics, Institute of Medicine, University of Tsukuba, Ibaraki.

Yoshimasa Imoto (Y)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Emiko Noguchi (E)

Department of Medical Genetics, Institute of Medicine, University of Tsukuba, Ibaraki. Electronic address: enoguchi@md.tsukuba.ac.jp.

Takako Nakamura (T)

Department of Medical Genetics, Institute of Medicine, University of Tsukuba, Ibaraki.

Wataru Morii (W)

Department of Medical Genetics, Institute of Medicine, University of Tsukuba, Ibaraki.

Naoto Adachi (N)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui; Department of Medical Genetics, Institute of Medicine, University of Tsukuba, Ibaraki.

Rieko Ii (R)

Department of Medical Genetics, Institute of Medicine, University of Tsukuba, Ibaraki.

Keisuke Koyama (K)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Satoshi Aoki (S)

Department of Otolaryngology, Dokkyo Medical University Saitama Medical Center, Saitama.

Keisuke Miyashita (K)

Department of Otolaryngology, Dokkyo Medical University Saitama Medical Center, Saitama.

Yu Hosokawa (Y)

Department of Otolaryngology, Dokkyo Medical University Saitama Medical Center, Saitama.

Kazuhiro Omura (K)

Department of Otolaryngology, Dokkyo Medical University Saitama Medical Center, Saitama.

Yasuhiro Tanaka (Y)

Department of Otolaryngology, Dokkyo Medical University Saitama Medical Center, Saitama.

Kaori Tanaka (K)

Division of Anaerobe Research, Life Science Research Center, Gifu University, Gifu.

Yukio Hida (Y)

Division of Clinical Laboratory, University of Fukui Hospital, Fukui.

Takahiro Ninomiya (T)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Yukinori Kato (Y)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Masafumi Sakashita (M)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Tetsuji Takabayashi (T)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Shigeharu Fujieda (S)

Division of Otorhinolaryngology and Head & Neck Surgery, Department of Sensory and Locomotor Medicine, Faculty of Medical Science, University of Fukui, Fukui.

Classifications MeSH