In vitro trypanocidal activity of extracts and compounds isolated from Vitellaria paradoxa.
In vitro
Trypanocidal activity
Trypanosoma brucei brucei
Vitellaria paradoxa
Journal
BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232
Informations de publication
Date de publication:
28 Sep 2023
28 Sep 2023
Historique:
received:
23
01
2023
accepted:
18
09
2023
medline:
5
10
2023
pubmed:
29
9
2023
entrez:
28
9
2023
Statut:
epublish
Résumé
Vitellaria paradoxa is used in traditional medicine for the treatment of various diseases in tropical countries; however, nothing is known about its anti-trypanosomal activity. Human African trypanosomiasis is a neglected tropical disease of Sub-Saharan Africa's poorest rural regions, and the efficacy of its treatment remains a challenge. This study investigates the as-yet-unknown trypanocidal activity of this plant. V. paradoxa, commonly known as shea tree, was selected for study based on an ethnobotanical investigation. Ultrasonicated extracts from bark and seeds were successively treated with ethyl acetate and water. Column chromatography, NMR spectroscopy and mass spectrometry were used to identify isolated compounds. Purified trypanosomes (Trypanosoma brucei brucei) were incubated with serial dilutions of the extracts and isolated compounds at 37 °C in 5% CO The ethyl acetate extracts of the bark showed the higher in vitro trypanocidal activity against T. brucei brucei with median inhibitory concentration (IC The results obtained contribute to the validation of the traditional medicinal use of V. paradoxa. Our results encourage further investigations of this plant, mainly with respect to its in vivo efficacy and toxicity.
Sections du résumé
BACKGROUND
BACKGROUND
Vitellaria paradoxa is used in traditional medicine for the treatment of various diseases in tropical countries; however, nothing is known about its anti-trypanosomal activity. Human African trypanosomiasis is a neglected tropical disease of Sub-Saharan Africa's poorest rural regions, and the efficacy of its treatment remains a challenge. This study investigates the as-yet-unknown trypanocidal activity of this plant.
METHODS
METHODS
V. paradoxa, commonly known as shea tree, was selected for study based on an ethnobotanical investigation. Ultrasonicated extracts from bark and seeds were successively treated with ethyl acetate and water. Column chromatography, NMR spectroscopy and mass spectrometry were used to identify isolated compounds. Purified trypanosomes (Trypanosoma brucei brucei) were incubated with serial dilutions of the extracts and isolated compounds at 37 °C in 5% CO
RESULTS
RESULTS
The ethyl acetate extracts of the bark showed the higher in vitro trypanocidal activity against T. brucei brucei with median inhibitory concentration (IC
CONCLUSION
CONCLUSIONS
The results obtained contribute to the validation of the traditional medicinal use of V. paradoxa. Our results encourage further investigations of this plant, mainly with respect to its in vivo efficacy and toxicity.
Identifiants
pubmed: 37770899
doi: 10.1186/s12906-023-04175-6
pii: 10.1186/s12906-023-04175-6
pmc: PMC10540432
doi:
Substances chimiques
ethyl acetate
76845O8NMZ
Plant Extracts
0
Trypanocidal Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
346Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
Références
Mishra AP, Sharifi-Rad M, Shariati MA, Mabkhot YN, Al-Showiman SS, Rauf A, Salehi B, Župunski M, Sharifi-Rad M, Gusain P. Bioactive compounds and health benefits of edible Rumex species-A review. Cell Mol Biol (Noisy-le-grand). 2018;64:27–34. https://doi.org/10.14715/cmb/2018.64.8.5 .
doi: 10.14715/cmb/2018.64.8.5
Sharifi-Rad M, Fokou PVT, Sharopov F, Martorell M, Ademiluyi AO, Rajkovic J, Salehi B, Martins N, Iriti M, Sharifi-Rad J. Antiulcer agents: from plant extracts to phytochemicals in healing promotion. Molecules. 2018;23: 1751.
doi: 10.3390/molecules23071751
pubmed: 30018251
pmcid: 6100067
Abbott RB, Hui KK, Hays RD, Mandel J, Goldstein M, Winegarden B, Glaser D, Brunton L. Medical student attitudes toward complementary, alternative and integrative medicine. Evidence-based complementary and alternative medicine. 2010;195:2011.
Cengiz Mordeniz. Introductory Chapter: Traditional and Complementary Medicine, Traditional and Complementary Medicine, Cengiz Mordeniz, IntechOpen, 2019, DOI: https://doi.org/10.5772/intechopen.86373 . Available from: https://www.intechopen.com/books/traditional-and-complementary-medicine/introductory-chapter-traditional-and-complementary-medicine . December 11th.
Mandal SC, Mandal V, Konishi T. Natural Products and Drug Discovery and integrated Approach. Elsevier; 2018.
World Health Organization. The World Traditional Medicines Situation, in traditional medicines: Global Situation, Issues and Challenges. Geneva. 2011;3:1–14.
Jansen O, Angenot L, Tits M, Nicolas JP, De Mol P, Nikiéma J-B, Frédérich M. Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties. J Ethnopharmacol. 2010;130:143–50. https://doi.org/10.1016/j.jep.2010.04.032 .
doi: 10.1016/j.jep.2010.04.032
pubmed: 20435124
WHO monographs on selected medicinal plants. WHO. 2009;4. https://apps.who.int/iris/handle/10665/42052 .
Kumar Patra J, Das G, Kumar S, Thatoi H. (Eds.). Ethnophamacology and Biodiversity of Medicinal plants (1st ed.). Apple Academic Press; 2019. https://doi.org/10.1201/9780429398193 .
Roberson E. Nature’s pharmacy, our treasure chest: why we must conserve our natural heritage, a native plant conservation campaign report. 2008. 16 p.
Tshimungu K, Okenge LN, Mukeba JN, Kande VB, De Mol P. Epidemiological, clinical and sociodemographic charateristics of human african trypanosomiasis(HAT) in and around Kinshasa, Democratic Republic of Congo. Sante. 2009;19(2):73–80.
pubmed: 20031514
Tong J, Valverde O, Mahoudeau C, Yun O, Chappuis F. Challenges of controlling sleeping sickness in areas of violent conflict: experience in the Democratic Republic of Congo. Confl Health. 2011;5: 7.
doi: 10.1186/1752-1505-5-7
pubmed: 21615932
pmcid: 3115864
Peter Babokhov, Adekunle O, Sanyaolu WA, Oyibo, Adetayo F, Fagbenro-Beyioku C, Iriemenam. A current analysis of chemotherapy strategies for the treatment of human african trypanosomiasis. Pathog Glob Health. 2013;107(5):242–52.
doi: 10.1179/2047773213Y.0000000105
Eller DT-DF. Kai Jensen, Shea (Vitellaria paradoxa C. F. Gaertn.) At the crossroads: current knowledge and research gaps. Agroforest Syst. 2018;92:1353–71.
doi: 10.1007/s10457-017-0080-y
Emmanuel Talla. Jean Noël Nyemb, Alembert Tchinda Tiabou, Sandrine Gael Zambou Djou, Pierre Biyanzi, Laurent Sophie, Luce Vander Elst and Joseph Mbafor Tanyi. Antioxidant Activity and a New Ursane-type Triterpene from Vitellaria paradoxa (Sapotaceae) Stem Barks. EJMP. 2016;16(3):1–20. https://doi.org/10.9734/EJMP/2016/28847 .
Eyong KO, Foyet HS, Baïrys G, Ngosong Folefoc G, Acha Asongalem E, Lagojda A, Lamshöft M. A new ursane triterpenoic acid and other potential anti-inflammatory and anti-arthritic constituents from EtOAc extracts of Vitellaria paradoxa stem bark. J Ethnopharmacol. 2015;174:277–86.
doi: 10.1016/j.jep.2015.08.014
pubmed: 26297841
Fodouop SP, Tala SD, Keilah LP, Kodjio N, Yemele MD, Kamdje Nwabo AH, Nji-Kah B, Tchoumboue J, Gatsing D. Effects of Vitellaria paradoxa (C.F. Gaertn.) Aqueous leaf extract administration on Salmonella typhimurium-infected rats. BMC Complement Altern Med. 2017;17(1):160.
doi: 10.1186/s12906-017-1643-1
pubmed: 28327127
pmcid: 5361855
Vincendeau P, Daëron M, Daulouede S. Identification of antibody classes and fc receptors responsible for phagocytosis of Trypanosoma musculi by mouse macrophages. Infect Immun. 1986;53(3):600–5.
doi: 10.1128/iai.53.3.600-605.1986
pubmed: 3744553
pmcid: 260833
Vincendeau P, Daëron M. Trypanosoma musculi co-express several receptors binding rodent IgM, IgE, and IgG subclasses. J Immunol. 1989;142(5):1702–9.
doi: 10.4049/jimmunol.142.5.1702
pubmed: 2918228
Kenneth O, Eyong G, Bairy AA, Eno J, Taube KG, Hull GN, Folefoc, Harquin S, Foyet. Daniel Romo, Triterpenoids from the stem bark of Vitellaria paradoxa (Sapotaceae) and derived esters exhibit cytotoxicity against a breast cancer cell line. Med Chem Res. 2018;27:268–77.
doi: 10.1007/s00044-017-2059-7
Bairy Guérisson. Étude phytochimique de Vitellaria Paradoxa c. f. Gaertn (Sapotaceae), transformations chimiques et activités biologiques contre les maladies neurodégénératives et le cancer, Thèse en Sciences, Université de Yaoundé I, 2022, 209. https://hdl.handle.net/20.500.12177/10137 .
Dauchy FA, Bonhivers M, Landrein N, Dacheux D, Courtois P, Lauruol F, Daulouède S, Vincendeau P, Robinson DR. Trypanosoma brucei CYP51: essentiality and targeting therapy in an experimental Model PLoS. Negl Trop Dis. 2016;10(11): e0005125.
doi: 10.1371/journal.pntd.0005125
Abrahamsohn IA, Silva WD. Antibody dependent cell-mediated cytotoxicity against Trypanosoma cruzi. Parasitology. 1977;75(3):317–23.
doi: 10.1017/S0031182000051866
pubmed: 415286
Mitsuko Ohashi M, Amoa-Bosompem KD, Kwofie J, Agyapong R, Adegle MM, Sakyiamah, et al. In vitro antiprotozoan activity and mechanisms of action of selected ghanaian medicinal plants against Trypanosoma, Leishmania, and Plasmodium parasites. Phytother Res. 2018;32(8):1617–30.
doi: 10.1002/ptr.6093
pubmed: 29733118
Mohammed A, Ibrahim AM, Musa AB, Aliyu, Hannah S, Mayaki A, Gideon. Md Shahidul Islam, Phenolics-rich fraction of Khaya senegalensis stem bark: antitrypanosomal activity and amelioration of some parasite-induced pathological changes. Pharm Biol. 2013;51(7):906–13.
doi: 10.3109/13880209.2013.771191
Abisoye LR. Antimalarial, Antitrypanosomal, Antimicrobial Activities and Volatile Oil Profile of Xylopia aethiopica (Dunal) Rich (Annonaceae). Letters in Applied NanoBioScience. 2022;11(3):3897–908.
Tchacondo T, Karou SD, Batawila K, Agban A, Ouro-Bang’na K, Anani KT, de Souza Gbeassor M. C. Herbal Remedies and Their Adverse Effects In Tem Tribe Traditional Medicine In Togo. African Journal of Traditional, Complementary and Alternative Medicines. 2011;8(1):45–60.
Nnadi CO. Solomon Onyedikachi Ngwu 1, Malunwanne Beatrice-Zita Ohagwu, In-Vivo and In-Silico Evidence of Antitrypanocidal Activities of Selected Plants from Asteraceae Family against Trypanosoma brucei brucei. Biointerface Research in Applied Chemistry. 2023;13(1): 30.
Djoueche CM, Azebaze AB, Dongmo AB. Investigation of plant for the Ethnoveterinary control of gastrointestinal parasites in Bénoué Region, Cameroon. Tropicultura. 2011;4:205–11.
Sudirman S, Chen CK, Long BT, Chang HW, Tsou D, Kong ZL. Vitellaria paradoxa Nut Triterpene-Rich Extract ameliorates symptoms of inflammation on post-traumatic osteoarthritis in obese rats. J Pain Res. 2020;13:261–71.
doi: 10.2147/JPR.S228766
pubmed: 32099450
pmcid: 6997037
Fodouop SP, Tala SD, Keilah LP, Kodjio N, Yemele MD, Nwabo Kamdje AH, Nji-Kah B, Tchoumboue J, Gatsing D. Effects of Vitellaria paradoxa (C.F. Gaertn.) aqueous leaf extract administration on Salmonella typhimurium-infected rats. BMC Complement Altern Med. 2017;17(1):160. https://doi.org/10.1186/s12906-017-1643-1 . Erratum in: BMC Complement Med Ther. 2023;23(1):40.
Sirignano C, Nadembega P, Poli F, Romano B, Lucariello G, Rigano D, Taglialatela-Scafati O. Triterpenoids from Vitellaria paradoxa stem barks reduce nitrite levels in LPS-Stimulated macrophages. Plants (Basel). 2021;10(5):1006.
doi: 10.3390/plants10051006
pubmed: 34070050
Ndukwe KC, Okeke IN, Lamikanra A, Adesina SK, Aboderin O. Antibacterial activity of aqueous extracts of selected chewing sticks. J Contemp Dent Pract. 2005;6(3):86–94.
doi: 10.5005/jcdp-6-3-86
pubmed: 16127476
Akihisa T, Kojima N, Katoh N, Kikuchi T, Fukatsu M, Shimizu N, Masters ET. Triacylglycerol and triterpene ester composition of shea nuts from seven african countries. J Oleo Sci. 2011;60:385–91.
doi: 10.5650/jos.60.385
pubmed: 21768739
Akihisa T, Kojima N, Kikuchi T, Yasukawa K, Tokuda H, Masters ET, Manosroi A, Manosroi J. Anti-inflammatory and chemopreventive effects of triterpene cinnamates and acetates from shea fat. J Oleo Sci. 2010;59:273–80.
doi: 10.5650/jos.59.273
pubmed: 20484832
Catteau L, Schioppa L, Beaufay C, Girardi C, Hérent MF, Frédérich M, Quetin-Leclercq J. Antiprotozoal activities of triterpenic acids and ester derivatives isolated from the Leaves of Vitellaria paradoxa. Planta Med. 2021;10–11:860–7.
Sut S, Dall’Acqua S, Baldan V, Ngahang Kamte SL, Ranjbarian F, Biapa Nya PC, Vittori S, Benelli G, Maggi F, Cappellacci L, Hofer A, Petrelli R. Identification of tagitinin C from Tithonia diversifolia as antitrypanosomal compound using bioactivity-guided fractionation. Fitoterapia. 2018;124:145–51.
doi: 10.1016/j.fitote.2017.11.002
pubmed: 29146170
Njogu M, Kimani JC, Matasyoh M, Kaiser R, Brun TJ, Schmidt. Sesquiterpene Lactones from Vernonia cinerascens Sch. Bip. and Their in Vitro Antitrypanosomal Activity, Molecules. 2018; 23 (2):248.
Steverding D, Sidjui LS, Ferreir ÉR, Ngameni B, Folefoc GN, Mahiou-Leddet V, Ollivier E, Stephenson GR, Storr TE, Tyler KM. T rypanocidal and leishmanicidal activity of six limonoids. J Nat Med. 2020;74(3):606–11.
doi: 10.1007/s11418-020-01408-7
pubmed: 32277328
pmcid: 7253382
Shiby M, Kuriakose R, Singh E. Uzonna host Intracellular Signaling events and pro-inflammatory cytokine production in african Trypanosomiasis. Front Immunol. 2016;7:181.
Okomo-Assoumou MC, Daulouede S, Lemesre JL, N’Zila-Mouanda A, Vincendeau P. Correlation of high serum levels of tumor necrosis factor-alpha with disease severity in human african trypanosomiasis. Am J Trop Med Hyg. 1995;53(5):539–43.
doi: 10.4269/ajtmh.1995.53.539
pubmed: 7485714
Vincendeau P, Bouteille B. Immunolgy and Immunopathology of African trypanosomiasis. An Acad Bras Ciênc. 2006;78(4):645–65.
doi: 10.1590/S0001-37652006000400004
pubmed: 17143404