Venom extraction method influences venom composition and potency in the giant house spider Eratigena atrica (C. L. Koch, 1843).
Composition
ED(50,) LD(50)
Extraction
Potency
Spider
Venom
Journal
Toxicon : official journal of the International Society on Toxinology
ISSN: 1879-3150
Titre abrégé: Toxicon
Pays: England
ID NLM: 1307333
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
29
06
2023
revised:
19
09
2023
accepted:
26
09
2023
pubmed:
30
9
2023
medline:
30
9
2023
entrez:
29
9
2023
Statut:
ppublish
Résumé
Extraction is the first step when investigating venom composition and function. In small invertebrates, widely used extraction methods include electrostimulation and venom gland extraction, however, the influence of these methods on composition and toxicology is poorly understood. Using the Giant House Spider Eratigena atrica as a model, we show that electrostimulation and venom gland removal extraction methods produce different protein profiles as assessed by Coomassie-stained SDS-PAGE and significantly different potencies in the cricket Acheta domesticus.
Identifiants
pubmed: 37775046
pii: S0041-0101(23)00289-1
doi: 10.1016/j.toxicon.2023.107303
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107303Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Keith Lyons reports financial support was provided by The Irish Research Council. Keith Lyons reports financial support was provided by the University of Galway, Thomas Crawford Hayes Grant Committee.