Cost-effective DNA methylation profiling by FML-seq.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
12 2023
12 2023
Historique:
received:
17
08
2023
revised:
20
09
2023
accepted:
21
09
2023
medline:
5
10
2023
pubmed:
30
9
2023
entrez:
29
9
2023
Statut:
epublish
Résumé
Current methods for profiling DNA methylation require costly reagents, sequencing, and labor time. We introduce fragmentation at methylated loci and sequencing (FML-seq), a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects.
Identifiants
pubmed: 37775270
pii: 6/12/e202302326
doi: 10.26508/lsa.202302326
pmc: PMC10546043
pii:
doi:
Substances chimiques
Fluorometholone
SV0CSG527L
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R01 CA193694
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023 Foley et al.
Références
Science. 2022 Apr;376(6588):44-53
pubmed: 35357919
Nat Commun. 2021 Feb 24;12(1):1286
pubmed: 33627650
Nature. 2008 Mar 13;452(7184):215-9
pubmed: 18278030
Genome Res. 2002 Jun;12(6):996-1006
pubmed: 12045153
Genome Res. 2018 Jan;28(1):88-99
pubmed: 29222086
Nucleic Acids Res. 2005 Oct 13;33(18):5868-77
pubmed: 16224102
Nucleic Acids Res. 2020 Jan 8;48(D1):D882-D889
pubmed: 31713622
BMC Genet. 2013 Jun 18;14:56
pubmed: 23773292
Genome Res. 2009 Jun;19(6):1044-56
pubmed: 19273619
Bioinformatics. 2017 Dec 15;33(24):3982-3984
pubmed: 28961746
J Clin Pathol. 2021 Nov;74(11):692-696
pubmed: 33431485
Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):11040-5
pubmed: 21690366
Genome Res. 2021 Jun 17;:
pubmed: 34140313
Nat Genet. 2005 Aug;37(8):853-62
pubmed: 16007088
PLoS One. 2021 May 11;16(5):e0247541
pubmed: 33974631
Genome Biol. 2014;15(12):550
pubmed: 25516281
Nucleic Acids Res. 2014 Jan;42(Database issue):D756-63
pubmed: 24259432
Plant Cell. 2009 Apr;21(4):1053-69
pubmed: 19376930
Science. 2022 Jun 17;376(6599):1257-1258
pubmed: 35709273
Nature. 2020 Jul;583(7818):699-710
pubmed: 32728249
J Bacteriol. 1975 Aug;123(2):768-70
pubmed: 1097428
Nature. 2012 Sep 6;489(7414):57-74
pubmed: 22955616
Nature. 2010 Jul 8;466(7303):253-7
pubmed: 20613842
Nat Biotechnol. 2009 Apr;27(4):361-8
pubmed: 19329998