SVep1, a temperate phage of human oral commensal
Siphoviridae family
Streptococcus vestibularis
lysogeny
oral phage
prophage engineering
temperature phage
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2023
2023
Historique:
received:
11
07
2023
accepted:
18
08
2023
medline:
2
10
2023
pubmed:
2
10
2023
entrez:
2
10
2023
Statut:
epublish
Résumé
Bacteriophages play a vital role in the human oral microbiome, yet their precise impact on bacterial physiology and microbial communities remains relatively understudied due to the limited isolation and characterization of oral phages. To address this gap, the current study aimed to isolate and characterize novel oral phages. To achieve this, oral bacteria were isolated using a culture-omics method from 30 samples collected from healthy individuals. These bacteria were then cultured in three different types of media under both aerobic and anaerobic conditions. The samples were subsequently subjected to full-length 16S rRNA gene sequencing for analysis. Subsequently, we performed the isolation of lytic and lysogenic phages targeting all these bacteria. In the initial step, a total of 75 bacterial strains were successfully isolated, representing 30 species and 9 genera. Among these strains, The presence of SVep1 may confer immunity to
Identifiants
pubmed: 37779698
doi: 10.3389/fmicb.2023.1256669
pmc: PMC10536254
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1256669Informations de copyright
Copyright © 2023 Wu, Zhu, Yang, Gong, Chen, Liao, Xiong, Zhou and Li.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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