The characterization of RNA-binding proteins and RNA metabolism-related proteins in fungal extracellular vesicles.
RNA metabolism
RNA-Binding Proteins
cellular communication
extracellular vesicles
proteomic data
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
25
06
2023
accepted:
23
08
2023
medline:
3
10
2023
pubmed:
2
10
2023
entrez:
2
10
2023
Statut:
epublish
Résumé
RNA-binding proteins (RBPs) are essential for regulating RNA metabolism, stability, and translation within cells. Recent studies have shown that RBPs are not restricted to intracellular functions and can be found in extracellular vesicles (EVs) in different mammalian cells. EVs released by fungi contain a variety of proteins involved in RNA metabolism. These include RNA helicases, which play essential roles in RNA synthesis, folding, and degradation. Aminoacyl-tRNA synthetases, responsible for acetylating tRNA molecules, are also enriched in EVs, suggesting a possible link between these enzymes and tRNA fragments detected in EVs. Proteins with canonical RNA-binding domains interact with proteins and RNA, such as the RNA Recognition Motif (RRM), Zinc finger, and hnRNP K-homology (KH) domains. Polyadenylate-binding protein (PABP) plays a critical role in the regulation of gene expression by binding the poly(A) tail of messenger RNA (mRNA) and facilitating its translation, stability, and localization, making it a key factor in post-transcriptional control of gene expression. The presence of proteins related to the RNA life cycle in EVs from different fungal species suggests a conserved mechanism of EV cargo packing. Various models have been proposed for selecting RNA molecules for release into EVs. Still, the actual loading processes are unknown, and further molecular characterization of these proteins may provide insight into the mechanism of RNA sorting into EVs. This work reviews the current knowledge of RBPs and proteins related to RNA metabolism in EVs derived from distinct fungi species, and presents an analysis of proteomic datasets through GO term and orthology analysis, Our investigation identified orthologous proteins in fungal EVs on different fungal species.
Identifiants
pubmed: 37780856
doi: 10.3389/fcimb.2023.1247329
pmc: PMC10539620
doi:
Substances chimiques
RNA
63231-63-0
RNA, Messenger
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1247329Informations de copyright
Copyright © 2023 Dallastella, Oliveira, Rodrigues, Goldenberg and Alves.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Sci Rep. 2018 Jul 17;8(1):10813
pubmed: 30018314
Nat Rev Mol Cell Biol. 2018 Apr;19(4):213-228
pubmed: 29339798
Clin Microbiol Rev. 2011 Oct;24(4):633-54
pubmed: 21976602
Semin Cancer Biol. 2014 Oct;28:3-13
pubmed: 24769058
Nat Rev Mol Cell Biol. 2019 Sep;20(9):509-510
pubmed: 31324871
Eukaryot Cell. 2007 Jan;6(1):48-59
pubmed: 17114598
Bioinformatics. 2006 Jul 1;22(13):1658-9
pubmed: 16731699
Comput Struct Biotechnol J. 2021 Apr 17;19:2286-2296
pubmed: 33995920
Cell Rep. 2016 Oct 11;17(3):799-808
pubmed: 27732855
J Biol Chem. 2013 Oct 11;288(41):29223-8
pubmed: 24003230
Elife. 2021 Nov 12;10:
pubmed: 34766549
Nucleic Acids Res. 2019 Jan 8;47(D1):D516-D519
pubmed: 30395310
Cell Microbiol. 2008 Aug;10(8):1695-710
pubmed: 18419773
J Clin Invest. 2005 Oct;115(10):2875-85
pubmed: 16151533
Infect Dis Clin North Am. 2002 Dec;16(4):837-74, v-vi
pubmed: 12512184
Acta Physiol (Oxf). 2018 Feb;222(2):
pubmed: 29253314
J Cell Sci. 2020 Sep 1;133(16):
pubmed: 32873715
Trends Biochem Sci. 2018 Apr;43(4):237-250
pubmed: 29486979
Elife. 2019 Aug 27;8:
pubmed: 31453808
Nat Plants. 2021 Mar;7(3):342-352
pubmed: 33633358
Front Microbiol. 2018 Oct 02;9:2286
pubmed: 30333803
J Extracell Vesicles. 2013 Dec 11;2:
pubmed: 24363837
Sci Rep. 2015 Jan 14;5:7763
pubmed: 25586039
RNA Biol. 2014;11(8):1019-30
pubmed: 25531407
PLoS One. 2010 Jun 14;5(6):e11113
pubmed: 20559436
Proteomics. 2019 Apr;19(8):e1800232
pubmed: 30883019
Wiley Interdiscip Rev RNA. 2014 Jul-Aug;5(4):461-80
pubmed: 24706556
mSphere. 2020 Aug 12;5(4):
pubmed: 32817450
Asian Pac J Cancer Prev. 2022 Jun 01;23(6):1893-1900
pubmed: 35763629
Fungal Biol Biotechnol. 2020 Sep 18;7:13
pubmed: 32968488
Clin Microbiol Rev. 2007 Jan;20(1):133-63
pubmed: 17223626
Med Sci Monit. 2020 Jun 05;26:e923210
pubmed: 32502142
Fungal Genet Biol. 2020 Nov;144:103447
pubmed: 32827756
Biochem Soc Trans. 2017 Dec 15;45(6):1313-1321
pubmed: 29150525
Sci Rep. 2017 Jan 04;7:39742
pubmed: 28051166
Cells. 2020 Apr 22;9(4):
pubmed: 32331346
Cell Mol Life Sci. 2017 Aug;74(16):2875-2897
pubmed: 28314893
Nat Commun. 2023 Jul 20;14(1):4383
pubmed: 37474601
J Extracell Vesicles. 2021 Aug;10(10):e12129
pubmed: 34377375
J Proteomics. 2014 Jan 31;97:177-86
pubmed: 23583696
Cell. 2016 Mar 10;164(6):1226-1232
pubmed: 26967288
PLoS Biol. 2012;10(12):e1001450
pubmed: 23271954
J Fungi (Basel). 2021 Jul 14;7(7):
pubmed: 34356940
Front Immunol. 2019 Feb 28;10:202
pubmed: 30873152
Nat Rev Genet. 2014 Dec;15(12):829-45
pubmed: 25365966
Nat Commun. 2013;4:2980
pubmed: 24356509
Int J Mol Sci. 2021 Sep 06;22(17):
pubmed: 34502537
J Extracell Vesicles. 2020 Dec;10(2):e12043
pubmed: 33391635
Front Cell Infect Microbiol. 2020 Nov 18;10:593160
pubmed: 33312966
Eukaryot Cell. 2014 Dec;13(12):1484-93
pubmed: 24906412
PLoS One. 2018 Apr 24;13(4):e0195969
pubmed: 29689087
Autophagy. 2023 May;19(5):1551-1561
pubmed: 36286616
mSphere. 2019 Mar 27;4(2):
pubmed: 30918062
Cells. 2021 Oct 06;10(10):
pubmed: 34685656
J Mol Med (Berl). 2022 May;100(5):679-695
pubmed: 35322869
Future Microbiol. 2011 Nov;6(11):1371-81
pubmed: 22082294
Eukaryot Cell. 2008 Jan;7(1):58-67
pubmed: 18039940
Nat Cell Biol. 2007 Jun;9(6):654-9
pubmed: 17486113
N Engl J Med. 2015 Oct 8;373(15):1445-56
pubmed: 26444731
Annu Rev Biochem. 2015;84:291-323
pubmed: 25784052
Biomolecules. 2023 May 18;13(5):
pubmed: 37238722
Cell Microbiol. 2015 Mar;17(3):389-407
pubmed: 25287304
Cold Spring Harb Perspect Biol. 2011 Jul 01;3(7):
pubmed: 21441581
Front Microbiol. 2022 Feb 18;13:817844
pubmed: 35250933
J Extracell Vesicles. 2015 May 14;4:27066
pubmed: 25979354
J Proteome Res. 2012 Mar 2;11(3):1676-85
pubmed: 22288420
Mycopathologia. 2020 Oct;185(5):813-842
pubmed: 32052359
Cold Spring Harb Perspect Biol. 2012 Sep 01;4(9):a012286
pubmed: 22763747
mSphere. 2016 Mar 30;1(2):
pubmed: 27303729
Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):505-16
pubmed: 21779027
Cell Rep. 2016 May 3;15(5):978-987
pubmed: 27117408
J Cell Biol. 2017 Jul 3;216(7):2201-2216
pubmed: 28611052
Medicine (Baltimore). 2000 Jul;79(4):250-60
pubmed: 10941354
Front Cell Infect Microbiol. 2019 Apr 11;9:100
pubmed: 31032233
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
pubmed: 9254694
Nat Commun. 2017 Feb 17;8:14448
pubmed: 28211508
PLoS Biol. 2018 Oct 8;16(10):e2006872
pubmed: 30296253
Elife. 2019 Aug 22;8:
pubmed: 31436530
Methods Mol Biol. 2016;1459:175-90
pubmed: 27665559
Methods Mol Biol. 2014;1097:491-521
pubmed: 24639174
J Extracell Vesicles. 2020 Nov;10(1):e12029
pubmed: 33708357
Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):E8987-E8995
pubmed: 29073095
J Proteome Res. 2015 Jan 2;14(1):142-53
pubmed: 25367658
Biochim Biophys Acta Proteins Proteom. 2019 Dec;1867(12):140203
pubmed: 30822540
Genes Dev. 2016 Mar 1;30(5):553-66
pubmed: 26944680
Front Plant Sci. 2020 Jan 10;10:1610
pubmed: 31998330
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712