Bioluminescent immunoassay for serine/threonine protein kinase activity using an aequorin-labeled monoclonal antibody and a synthetic peptide as a substrate.
Aurora B kinase
Chemical conjugation
Immunoassay
Luciferase
Photoprotein
Protein kinase inhibitor
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
12 11 2023
12 11 2023
Historique:
received:
30
08
2023
accepted:
21
09
2023
medline:
23
10
2023
pubmed:
2
10
2023
entrez:
2
10
2023
Statut:
ppublish
Résumé
A bioluminescent immunoassay system was developed to determine serine/threonine protein kinase activity using an aequorin-labeled monoclonal antibody and a synthetic peptide as the substrate. A monoclonal antibody against the synthetic phosphorylated serine peptide (K9P peptide) of histone H3 (19 amino acid residues), referred to as the H3S10P antibody, was chemically conjugated to maleimide-activated aequorin to prepare aequorin-labeled H3S10P (AQ-S-H3S10P). For the serine/threonine kinase assay, a non-phosphorylated serine peptide (K9C peptide) coated on a microplate was incubated with serine/threonine protein kinase in the presence of ATP and Mg
Identifiants
pubmed: 37783115
pii: S0006-291X(23)01098-7
doi: 10.1016/j.bbrc.2023.09.062
pii:
doi:
Substances chimiques
Aequorin
50934-79-7
Antibodies, Monoclonal
0
Peptides
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
Protein Kinases
EC 2.7.-
Threonine
2ZD004190S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
180-185Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest J. Sato and S. Inouye are employees in JNC Corporation. H. Onogi, and N. Nomura are employees in KinoPharma, Inc. M. Hagiwara declares that there are no conflicts of interest.