Testing of worn face mask and saliva for SARS-CoV-2.
COVID-19
SARS-CoV-2
hemodialysis
immunocompromised
viral shedding
Journal
Frontiers in public health
ISSN: 2296-2565
Titre abrégé: Front Public Health
Pays: Switzerland
ID NLM: 101616579
Informations de publication
Date de publication:
2023
2023
Historique:
received:
15
06
2023
accepted:
04
09
2023
medline:
1
11
2023
pubmed:
4
10
2023
entrez:
4
10
2023
Statut:
epublish
Résumé
Exhaled SARS-CoV-2 can be detected on face masks. We compared tests for SARS-CoV-2 RNA on worn face masks and matched saliva samples. We conducted this prospective, observational, case-control study between December 2021 and March 2022. Mask and saliva testing specificities were 99% and 100%, respectively. Test sensitivity was 62% for masks, and 81% for saliva ( While SARS-CoV-2 testing on worn masks appears to be less sensitive compared to saliva, it may be a preferred screening method for individuals who are mandated to wear masks yet averse to more invasive sampling. However, optimized RNA extraction methods and automated procedures are warranted to increase test sensitivity and scalability. We corroborated longer viral RNA shedding in immunocompromised patients.
Sections du résumé
Background
Exhaled SARS-CoV-2 can be detected on face masks. We compared tests for SARS-CoV-2 RNA on worn face masks and matched saliva samples.
Methods
We conducted this prospective, observational, case-control study between December 2021 and March 2022.
Results
Mask and saliva testing specificities were 99% and 100%, respectively. Test sensitivity was 62% for masks, and 81% for saliva (
Conclusion
While SARS-CoV-2 testing on worn masks appears to be less sensitive compared to saliva, it may be a preferred screening method for individuals who are mandated to wear masks yet averse to more invasive sampling. However, optimized RNA extraction methods and automated procedures are warranted to increase test sensitivity and scalability. We corroborated longer viral RNA shedding in immunocompromised patients.
Identifiants
pubmed: 37790720
doi: 10.3389/fpubh.2023.1237512
pmc: PMC10545089
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Observational Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1237512Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK130067
Pays : United States
Informations de copyright
Copyright © 2023 Wang, Thwin, Haq, Dong, Tisdale, Fuentes, Grobe and Kotanko.
Déclaration de conflit d'intérêts
XW, OT, ZH, ZD, LT, LF, NG, and PK are employees of the Renal Research Institute, a wholly owned subsidiary of Fresenius Medical Care. PK holds stock in Fresenius Medical Care.
Références
Eur J Clin Microbiol Infect Dis. 2021 Dec;40(12):2489-2496
pubmed: 34224033
PLoS One. 2022 Aug 18;17(8):e0270765
pubmed: 35980889
N Engl J Med. 2020 Sep 24;383(13):1283-1286
pubmed: 32857487
Sci Rep. 2021 Jun 29;11(1):13476
pubmed: 34188082
Clin Infect Dis. 2021 Sep 15;73(6):e1397-e1401
pubmed: 33949644
J Am Soc Nephrol. 2021 Nov;32(11):2728-2730
pubmed: 34281959
J Dent Res. 2020 Dec;99(13):1435-1443
pubmed: 32936047
J Infect Dis. 2022 Nov 11;226(10):1726-1730
pubmed: 36134610
Clin Infect Dis. 2023 Feb 8;76(3):e148-e154
pubmed: 35870128
N Engl J Med. 2022 Jul 21;387(3):275-277
pubmed: 35767428
J Infect. 2021 Jun;82(6):253-259
pubmed: 33774019
Am J Infect Control. 2022 Aug;50(8):890-897
pubmed: 35908828
Int J Infect Dis. 2022 Oct;123:25-33
pubmed: 35932968