Imaging and component analysis of pumpkin stem tissue with simultaneous SF-CARS and TPEF microscopy.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Sep 2023
01 Sep 2023
Historique:
received:
05
06
2023
revised:
23
07
2023
accepted:
16
08
2023
medline:
4
10
2023
pubmed:
4
10
2023
entrez:
4
10
2023
Statut:
epublish
Résumé
A multimodal nonlinear optical imaging platform based on a single femtosecond oscillator is built for simultaneous TPEF and SF-CARS imaging. TPEF microscopy and SF-CARS microscopy is utilized for mapping the distribution of the lignin component and the polysaccharide component, respectively. Visualization of vessel structure is realized. And the relative distribution of lignin and polysaccharide of vessel structure is mapped. Two pumpkin stem tissue areas with different degrees of lignification are observed with simultaneous TPEF and SF-CARS imaging, and two types of cell walls are identified. The different distribution patterns of lignin and polysaccharide in these two types of cell walls, induced by different degrees of lignification, are analyzed in detail.
Identifiants
pubmed: 37791252
doi: 10.1364/BOE.497260
pii: 497260
pmc: PMC10545196
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4862-4874Informations de copyright
© 2023 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
Opt Lett. 2015 Nov 15;40(22):5204-7
pubmed: 26565835
Front Bioeng Biotechnol. 2020 Oct 09;8:585363
pubmed: 33163482
J Biophotonics. 2014 Jan;7(1-2):49-58
pubmed: 23242840
J Biomed Opt. 2011 Feb;16(2):021112
pubmed: 21361675
J Phys Chem A. 2019 May 2;123(17):3928-3934
pubmed: 30957999
Opt Lett. 2017 Sep 15;42(18):3634-3637
pubmed: 28914920
Opt Lett. 2017 Apr 15;42(8):1548-1551
pubmed: 28409794
Polymers (Basel). 2022 Feb 28;14(5):
pubmed: 35267784
Opt Express. 2020 Jul 6;28(14):20794-20807
pubmed: 32680132
J Biophotonics. 2021 Jun;14(6):e202100040
pubmed: 33720518
Opt Express. 2018 May 28;26(11):14307-14314
pubmed: 29877471
Analyst. 2021 Apr 7;146(7):2277-2291
pubmed: 33617612
J Biomed Opt. 2020 Jan;25(1):1-12
pubmed: 32003191
Opt Lett. 2016 Jun 1;41(11):2628-31
pubmed: 27244431
Opt Express. 2018 Apr 16;26(8):10230-10241
pubmed: 29715963
Opt Express. 2009 Feb 16;17(4):2984-96
pubmed: 19219203
J Biophotonics. 2019 Jun;12(6):e201800052
pubmed: 30597770
Trends Plant Sci. 2019 Sep;24(9):867-878
pubmed: 31257154
Biomed Opt Express. 2016 Sep 27;7(10):4335-4345
pubmed: 27867735
Nano Lett. 2018 Feb 14;18(2):1366-1372
pubmed: 29376374
Science. 2004 Dec 24;306(5705):2206-11
pubmed: 15618507
Opt Express. 2009 Nov 9;17(23):20700-6
pubmed: 19997300
Front Pharmacol. 2021 Feb 03;12:630167
pubmed: 33613294