A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.
A2A adenosine receptor
archaerhodopsin-3
lipidic cubic phase
membrane proteins
serial crystallography
structure-based drug design
Journal
Journal of applied crystallography
ISSN: 0021-8898
Titre abrégé: J Appl Crystallogr
Pays: United States
ID NLM: 9876190
Informations de publication
Date de publication:
01 Oct 2023
01 Oct 2023
Historique:
received:
06
03
2023
accepted:
24
07
2023
medline:
4
10
2023
pubmed:
4
10
2023
entrez:
4
10
2023
Statut:
epublish
Résumé
Serial crystallography has emerged as an important tool for structural studies of integral membrane proteins. The ability to collect data from micrometre-sized weakly diffracting crystals at room temperature with minimal radiation damage has opened many new opportunities in time-resolved studies and drug discovery. However, the production of integral membrane protein microcrystals in lipidic cubic phase at the desired crystal density and quantity is challenging. This paper introduces VIALS (versatile approach to high-density microcrystals in lipidic cubic phase for serial crystallography), a simple, fast and efficient method for preparing hundreds of microlitres of high-density microcrystals suitable for serial X-ray diffraction experiments at both synchrotron and free-electron laser sources. The method is also of great benefit for rational structure-based drug design as it facilitates
Identifiants
pubmed: 37791355
doi: 10.1107/S1600576723006428
pii: S1600576723006428
pmc: PMC10543674
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1361-1370Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 210734/Z/18/Z
Pays : United Kingdom
Informations de copyright
© James Birch et al. 2023.
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