Three alginate lyases provide a new gut

Bacteroides ovatus Bacteroidetes alginate lyase crystal structure genome mining metagenomic gene catalog mode of action polysaccharide utilization loci proteome analysis synergy

Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
31 10 2023
Historique:
medline: 1 11 2023
pubmed: 4 10 2023
entrez: 4 10 2023
Statut: ppublish

Résumé

Humans consume alginate in the form of seaweed, food hydrocolloids, and encapsulations, making the digestion of this mannuronic acid (M) and guluronic acid (G) polymer of key interest for human health. To increase knowledge on alginate degradation in the gut, a gene catalog from human feces was mined for potential alginate lyases (ALs). The predicted ALs were present in nine species of the Bacteroidetes phylum, of which two required supplementation of an

Identifiants

pubmed: 37791757
doi: 10.1128/aem.01185-23
pmc: PMC10617595
doi:

Substances chimiques

poly(beta-D-mannuronate) lyase EC 4.2.2.3
Alginates 0
Oligosaccharides 0
Polysaccharide-Lyases EC 4.2.2.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0118523

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

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Auteurs

Mette E Rønne (ME)

Department of Biotechnology and Biomedicine, Enzyme and Protein Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark.

Tobias Tandrup (T)

Department of Biotechnology and Biomedicine, Enzyme and Protein Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark.

Mikkel Madsen (M)

Department of Biotechnology and Biomedicine, Enzyme and Protein Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark.

Cameron J Hunt (CJ)

Department of Biotechnology and Biomedicine, Enzyme Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Pernille N Myers (PN)

Department of Biotechnology and Biomedicine, Disease Systems Immunology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Janne M Moll (JM)

Department of Biotechnology and Biomedicine, Disease Systems Immunology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Jesper Holck (J)

Department of Biotechnology and Biomedicine, Enzyme Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Susanne Brix (S)

Department of Biotechnology and Biomedicine, Disease Systems Immunology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Mikael L Strube (ML)

Department of Biotechnology and Biomedicine, Bacterial Ecophysiology and Biotechnology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Finn L Aachmann (FL)

Department of Biotechnology and Food Science, Norwegian Biopolymer Laboratory (NOBIPOL), NTNU Norwegian University of Science and Technology, Trondheim, Norway.

Casper Wilkens (C)

Department of Biotechnology and Biomedicine, Enzyme Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.
Department of Biotechnology and Biomedicine, Structural Enzymology, Technical University of Denmark, Kgs. Lyngby, Denmark.

Birte Svensson (B)

Department of Biotechnology and Biomedicine, Enzyme and Protein Chemistry, Technical University of Denmark, Kgs. Lyngby, Denmark.

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Classifications MeSH