OptoPi: An open source flexible platform for the analysis of small animal behaviour.
3D-printing
Arduino
Behaviour
LED
Laser cutting
Light stimulation
Neuroscience
Open hardware
Optogenetics
Journal
HardwareX
ISSN: 2468-0672
Titre abrégé: HardwareX
Pays: England
ID NLM: 101710262
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
13
07
2022
revised:
24
02
2023
accepted:
11
06
2023
medline:
5
10
2023
pubmed:
5
10
2023
entrez:
5
10
2023
Statut:
epublish
Résumé
Behaviour is the ultimate output of neural circuit computations, and therefore its analysis is a cornerstone of neuroscience research. However, every animal and experimental paradigm requires different illumination conditions to capture and, in some cases, manipulate specific behavioural features. This means that researchers often develop, from scratch, their own solutions and experimental set-ups. Here, we present OptoPi, an open source, affordable (∼ £600), behavioural arena with accompanying multi-animal tracking software. The system features highly customisable and reproducible visible and infrared illumination and allows for optogenetic stimulation. OptoPi acquires images using a Raspberry Pi camera, features motorised LED-based illumination, Arduino control, as well as irradiance monitoring to fine-tune illumination conditions with real time feedback. Our open-source software (BioImageProcessing) can be used to simultaneously track multiple unmarked animals both in on-line and off-line modes. We demonstrate the functionality of OptoPi by recording and tracking under different illumination conditions the spontaneous behaviour of larval zebrafish as well as adult
Identifiants
pubmed: 37795340
doi: 10.1016/j.ohx.2023.e00443
pii: S2468-0672(23)00050-0
pmc: PMC10545942
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00443Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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