Comparative biological properties of resin-free and resin-based calcium silicate-based endodontic repair materials on human periodontal ligament stem cells.
Calcium ion release
Calcium silicate-based sealers
Cell migration
Cytocompatibility
Periodontal ligament stem cells
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
04
05
2023
accepted:
27
09
2023
medline:
8
11
2023
pubmed:
5
10
2023
entrez:
5
10
2023
Statut:
ppublish
Résumé
To investigate the effect of three different calcium silicate-based materials (CSBM) on the biological behavior of human periodontal ligament stem cells (hPDLSCs). Eluates of Biodentine, NeoPutty and TheraCal PT prepared at 1:1, 1:2, and 1:4 ratios were extracted under sterile conditions. The cytotoxicity of the extracts to the hPDLSCs was assessed using the MTT assay. Scratch wound healing assay was utilized for assessing cell migration. Scanning electron microscopy was used to detect cell attachment and morphology. Calcium ion release was measured using inductively coupled plasma-optical emission spectrometry; the pH-value was evaluated with a pH-meter. ANOVA with post hoc Tukey test was used for statistical analysis. Cell viability was significantly higher for Biodentine and NeoPutty at day 1 with all dilutions (p < 0.05), while at day 3 and day 7 with dilutions 1:2 and 1:4; all materials showed similar behavior (p > 0.05). Biodentine had the highest percentage of cell migration into the scratched area at day 1 for all dilutions (p < 0.05). Stem cells were attached favorably on Biodentine and NeoPutty with evident spreading, and intercellular communications; however, this was not shown for TheraCal PT. Biodentine showed the highest pH values and calcium ion release (p < 0.05). The resin-free CSBM showed better performance and favorable biological effects on hPDLSCs and were therefore considered promising for usage as endodontic repair materials. Proper selection of materials with favorable impact on the host stem cells is crucial to ensure outcome in different clinical scenarios.
Identifiants
pubmed: 37796335
doi: 10.1007/s00784-023-05288-5
pii: 10.1007/s00784-023-05288-5
pmc: PMC10630253
doi:
Substances chimiques
tricalcium silicate
404G39282C
calcium silicate
S4255P4G5M
TheraCal
0
Repair Material
0
Root Canal Filling Materials
0
Calcium
SY7Q814VUP
Calcium Compounds
0
Silicates
0
Oxides
0
Types de publication
Journal Article
Langues
eng
Pagination
6757-6768Informations de copyright
© 2023. The Author(s).
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