Mycothiol maintains the homeostasis and signalling of nitric oxide in Streptomyces coelicolor A3(2) M145.

Streptomyces coelicolor / genetics Nitric Oxide / metabolism Cysteine / metabolism Homeostasis Gene Expression Regulation, Bacterial Anthraquinones / metabolism Bacterial Proteins / genetics Anti-Bacterial Agents / pharmacology
Actinobacteria Intracellular homeostasis Mycothiol Nitric oxide Secondary metabolism Signal transduction

Journal

BMC microbiology
ISSN: 1471-2180
Titre abrégé: BMC Microbiol
Pays: England
ID NLM: 100966981

Informations de publication

Date de publication:
05 10 2023
Historique:
received: 08 12 2022
accepted: 01 10 2023
medline: 9 10 2023
pubmed: 6 10 2023
entrez: 5 10 2023
Statut: epublish

Résumé

Previous studies have revealed a nitric oxide (NO) metabolic cycle in which NO, nitrate (NO To investigate the interaction of MSH with endogenously produced NO, we generated an S. coelicolor A3(2) strain deficient in MSH biosynthesis. This mutant strain exhibited a decrease in low-molecular-weight S-nitrosothiols and intracellular NO levels during culture compared to those of the wild-type strain. Moreover, the mutant strain exhibited reduced activity of the DevS/DevR TCS, a regulator of NO homeostasis and ACT production, from the early stage of culture, along with a decrease in ACT production compared to those of the wild-type strain. This study suggests that MSH maintains intracellular NO homeostasis by forming S-nitrosomycothiol, which induces NO signalling. Finally, we propose a metabolic model in which MSH from endogenously produced NO facilitates the maintenance of both NO homeostasis and signalling in S. coelicolor A3(2) M145.

Sections du résumé

BACKGROUND
Previous studies have revealed a nitric oxide (NO) metabolic cycle in which NO, nitrate (NO
RESULTS
To investigate the interaction of MSH with endogenously produced NO, we generated an S. coelicolor A3(2) strain deficient in MSH biosynthesis. This mutant strain exhibited a decrease in low-molecular-weight S-nitrosothiols and intracellular NO levels during culture compared to those of the wild-type strain. Moreover, the mutant strain exhibited reduced activity of the DevS/DevR TCS, a regulator of NO homeostasis and ACT production, from the early stage of culture, along with a decrease in ACT production compared to those of the wild-type strain.
CONCLUSIONS
This study suggests that MSH maintains intracellular NO homeostasis by forming S-nitrosomycothiol, which induces NO signalling. Finally, we propose a metabolic model in which MSH from endogenously produced NO facilitates the maintenance of both NO homeostasis and signalling in S. coelicolor A3(2) M145.

Identifiants

DOI: 10.1186/s12866-023-03036-z PMID: 37798648 PMC: PMC10552308
pubmed: 37798648
doi: 10.1186/s12866-023-03036-z
pii: 10.1186/s12866-023-03036-z
pmc: PMC10552308
doi:

Substances chimiques

mycothiol 0
Nitric Oxide 31C4KY9ESH
Cysteine K848JZ4886
Anthraquinones 0
Bacterial Proteins 0
Anti-Bacterial Agents 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

285

Informations de copyright

© 2023. BioMed Central Ltd., part of Springer Nature.

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Auteurs

Tomoki Yoshizumi (T)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan.

Yukiko Shibui (Y)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan.

Minori Kogo (M)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan.

Sota Honma (S)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan.

Shinsaku Ito (S)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan.

Shunsuke Yajima (S)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan.

Yasuyuki Sasaki (Y)

Department of Bioscience, Faculty of Life Sciences, Tokyo University of Agriculture, Sakuragaoka, Tokyo, Setagaya-Ku, 156-8502, Japan. y1sasaki@nodai.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Streptomycetaceae › Streptomyces › Streptomyces coelicolor › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Oxydes d'azote › Monoxyde d'azote › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés soufrés › Cystéine › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Phénomènes physiologiques › Homéostasie › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Anthracènes › Anthraquinones › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Mycothiol maintains the homeostasis and...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Mycothiol maintains the homeostasis and...