Expanding the toolbox of probiotic Escherichia coli Nissle 1917 for synthetic biology.
Escherichia coli Nissle 1917
cell-free protein synthesis
cryptic plasmid
integrase
synthetic biology
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
06 Oct 2023
06 Oct 2023
Historique:
revised:
11
09
2023
received:
06
07
2023
accepted:
27
09
2023
pubmed:
6
10
2023
medline:
6
10
2023
entrez:
6
10
2023
Statut:
aheadofprint
Résumé
Escherichia coli Nissle 1917 (EcN) is a probiotic microbe that has the potential to be developed as a promising chassis for synthetic biology applications. However, the molecular tools and techniques for utilizing EcN remain to be further explored. To address this opportunity, the EcN-based toolbox was systematically expanded, enabling EcN as a powerful platform for more applications. First, two EcN cryptic plasmids and other compatible plasmids were genetically engineered to enrich the manipulable plasmid toolbox for multiple gene coexpression. Next, two EcN-based technologies were developed, including the conjugation strategy for DNA transfer, and quantification of protein expression capability. Finally, the EcN-based applications were further expanded by developing EcN native integrase-mediated genetic engineering and establishing an in vitro cell-free protein synthesis (CFPS) system. Overall, this study expanded the toolbox for manipulating and making full use of EcN as a commonly used probiotic chassis, providing several simplified, dependable, and predictable strategies for researchers working in synthetic biology fields.
Identifiants
pubmed: 37800393
doi: 10.1002/biot.202300327
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300327Subventions
Organisme : National Natural Science Foundation of China
ID : 32171427
Organisme : National Natural Science Foundation of China
ID : 31971348
Informations de copyright
© 2023 Wiley-VCH GmbH.
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