Mechanical stress confers nuclear and functional changes in derived leukemia cells from persistent confined migration.
Cell migration
DNA damage
Lamin
Mechanobiology
Nuclear deformability
Journal
Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402
Informations de publication
Date de publication:
06 Oct 2023
06 Oct 2023
Historique:
received:
14
03
2023
accepted:
14
09
2023
revised:
07
08
2023
medline:
26
10
2023
pubmed:
6
10
2023
entrez:
6
10
2023
Statut:
epublish
Résumé
Nuclear deformability plays a critical role in cell migration. During this process, the remodeling of internal components of the nucleus has a direct impact on DNA damage and cell behavior; however, how persistent migration promotes nuclear changes leading to phenotypical and functional consequences remains poorly understood. Here, we described that the persistent migration through physical barriers was sufficient to promote permanent modifications in migratory-altered cells. We found that derived cells from confined migration showed changes in lamin B1 localization, cell morphology and transcription. Further analysis confirmed that migratory-altered cells showed functional differences in DNA repair, cell response to chemotherapy and cell migration in vivo homing experiments. Experimental modulation of actin polymerization affected the redistribution of lamin B1, and the basal levels of DNA damage in migratory-altered cells. Finally, since major nuclear changes were present in migratory-altered cells, we applied a multidisciplinary biochemical and biophysical approach to identify that confined conditions promoted a different biomechanical response of the nucleus in migratory-altered cells. Our observations suggest that mechanical compression during persistent cell migration has a role in stable nuclear and genomic alterations that might handle the genetic instability and cellular heterogeneity in aging diseases and cancer.
Identifiants
pubmed: 37801090
doi: 10.1007/s00018-023-04968-5
pii: 10.1007/s00018-023-04968-5
pmc: PMC10558412
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
316Subventions
Organisme : Agencia Estatal de Investigación
ID : SAF2017-86327-R
Organisme : Agencia Estatal de Investigación
ID : PDI2020-118525RB-I00
Organisme : Agencia Estatal de Investigación
ID : RTI2018-097267-B-I00
Organisme : Agencia Estatal de Investigación
ID : FPI Scholarship 2018
Organisme : Agencia Estatal de Investigación
ID : PID2020-115444GB-I00
Organisme : Agencia Estatal de Investigación
ID : PID2019-108391RB-100
Organisme : Agencia Estatal de Investigación
ID : TED2021-132296B-C52
Organisme : Fundación BBVA
ID : Beca Leonardo 2020
Organisme : Consejería de Educación, Juventud y Deporte, Comunidad de Madrid
ID : PEJ-2020-AI/BMD-19152
Organisme : Consejería de Educación, Juventud y Deporte, Comunidad de Madrid
ID : Y2018/BIO-5207
Organisme : Consejería de Educación, Juventud y Deporte, Comunidad de Madrid
ID : Y2018/BIO-5207
Organisme : Consejería de Educación, Juventud y Deporte, Comunidad de Madrid
ID : Y2018/BIO-5207
Organisme : Fundación Fero
ID : BFERO2021.01
Organisme : Fundación Científica Asociación Española Contra el Cáncer
ID : LABAE211656TORR
Organisme : Comunidad de Madrid
ID : S2018/NMT-4389
Organisme : Comunidad de Madrid
ID : REACT-EU program PR38-21-28 ANTICIPA-CM
Organisme : Fundación General CSIC
ID : JAE Intro 2022
Organisme : Fundación General CSIC
ID : JAE Intro 2022
Informations de copyright
© 2023. The Author(s).
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