Use of high frequency electrorotation to identify cytoplasmic changes in cells non-disruptively.
Cell-analysis
Electrorotation
Non-disruptive analysis, single-cell analysis
Journal
Biomedical microdevices
ISSN: 1572-8781
Titre abrégé: Biomed Microdevices
Pays: United States
ID NLM: 100887374
Informations de publication
Date de publication:
06 10 2023
06 10 2023
Historique:
accepted:
12
09
2023
medline:
9
10
2023
pubmed:
6
10
2023
entrez:
6
10
2023
Statut:
epublish
Résumé
In this paper we demonstrate how the use of frequencies ranging from 50 kHz to 5 GHz in the analysis of cells by electrorotation can open the path to the identification of differences not detectable by conventional set-ups. Earlier works usually reported electrorotation devices operating below 20 MHz, limiting the response obtained to properties associated with the cell membrane. Those devices are thus unable to resolve the physiological properties in the cytoplasm. We used microwave-based technology to extend the frequency operation to 5 GHz. At high frequencies (from tens of MHz to GHz), the electromagnetic signal passes through the membrane and allows probing the cytoplasm. This enables several applications, such as cell classification, and viability analysis. Additionally, the use of conventional microfabrication techniques reduces the cost and complexity of analysis, compared to other non-invasive methods. We demonstrated the potential of this set-up by identifying two different populations of T-lymphocytes not distinguishable through visual assessment. We also assessed the effect of calcein on cell cytoplasmic properties and used it as a controlled experiment to demonstrate the possibility of this method to detect changes happening predominantly in the cytoplasm.
Identifiants
pubmed: 37801137
doi: 10.1007/s10544-023-00677-9
pii: 10.1007/s10544-023-00677-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
39Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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