Neuroprotective Effect of Ethanolic Extract of Scoparia dulcis on Acrylamide-Induced Neurotoxicity in Zebrafish Model.
Acrylamide
HSP-70
Neuroprotective
Scoparia dulcis
T-maze
Zebrafish
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
06 Oct 2023
06 Oct 2023
Historique:
accepted:
15
09
2023
medline:
6
10
2023
pubmed:
6
10
2023
entrez:
6
10
2023
Statut:
aheadofprint
Résumé
All herbal medicines are reported to be safe and have better results in curing disabilities. Scoparia dulcis is known for its anti-inflammatory and antioxidant properties. This study has been executed to explore the neuroprotective effects of ethanolic extract of Scoparia dulcis (EESD) against acrylamide using adult zebrafish. The experimental period was 72 h. After fixing the optimum acrylamide concentration and EESD, the healthy adult fish were grouped into control, induction, and treatment. During the experimental period, behavioural changes such as memory and locomotion were observed in control and experimental groups using the T-maze experiment. After 72 h, the neuronal tissues were isolated from the grouped fishes and analysed for various biochemical and enzymatic assays. The mRNA of the HSP-70 gene in control and experimental groups was expressed using RT-PCR. The optimum dosages for acrylamide and EESD were found to be 0.75 mM and 20 µg/mL, respectively. Memory improvement was observed in S. dulcis-treated fish, compared to the acrylamide-treated group using the T-maze assay. The extract reduced the toxicity induced by acrylamide from the various biochemical and histopathological parameters. The result shows the potential neuroprotective effects of ethanolic extract of Scoparia dulcis (EESD) against acrylamide-induced neurotoxicity in adult zebrafish. Therefore, Scoparia dulcis is a potent neuroprotective agent.
Identifiants
pubmed: 37801272
doi: 10.1007/s12010-023-04733-1
pii: 10.1007/s12010-023-04733-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
LoPachin, R. M., & Gavin, T. (2012). Molecular mechanism of acrylamide neurotoxicity: Lessons learned from organic chemistry. Environmental Health Perspectives, 120(12), 1650–1657.
doi: 10.1289/ehp.1205432
pubmed: 23060388
pmcid: 3548275
Singh, K. G., Keerthana, P., & Johnson, S. (2019). Neuroprotective effect of Mulberry (Morus nigra) leaf extract on acrylamide–induced Zebrafish (Danio rerio). International Journal Pharmaceutical Sciences and Research, 10(1), 12–15.
Parng, C., Roy, N. M., Ton, C., Lin, Y., & McGrath, P. (2007). Neurotoxicity assessment using zebrafish. Journal of pharmacological and toxicological methods, 55(1), 103–112.
doi: 10.1016/j.vascn.2006.04.004
pubmed: 16769228
Park, J. S., Samanta, P., Lee, S., Lee, J., Cho, J. W., Chun, H. S., ... & Kim, W. K. (2021). Developmental and neurotoxicity of acrylamide to zebrafish. International journal of molecular sciences, 22(7), 3518.
Erkekoglu, P., & Baydar, T. (2014). Acrylamide neurotoxicity. Nutritional neuroscience, 17(2), 49–57.
doi: 10.1179/1476830513Y.0000000065
pubmed: 23541332
Faria, M., Valls, A., Prats, E., Bedrossiantz, J., Orozco, M., Porta, J. M., ... & Raldúa, D. (2019). Further characterization of the zebrafish model of acrylamide acute neurotoxicity: Gait abnormalities and oxidative stress. Scientific reports, 9(1) 7075.
Kachot, R. L., Patel, U. D., Patel, H. B., Modi, C. M., Chauhan, R., Kariya, M. H., & Bhadaniya, A. R. (2023). Neurotoxicity of acrylamide in adult zebrafish following short-term and long-term exposure: Evaluation of behavior alterations, oxidative stress markers, expression of antioxidant genes, and histological examination of the brain and eyes. Environmental Science and Pollution Research, 1–16.
Ngoc Hieu, B. T., Ngoc Anh, N. T., Audira, G., Juniardi, S., Liman, R., Villaflores, O. B., Lai, Y. H., Chen, J. R., Liang, S. T., Huang, J. C., & Hsiao, C. D. (2020). Development of a modified three-day T-maze protocol for evaluating learning and memory capacity of adult zebrafish. International journal of molecular sciences, 21(4), 1464.
doi: 10.3390/ijms21041464
pubmed: 32098080
pmcid: 7073029
Pawar, Vikas & Sarawade, Rachana. (2020). Neuroprotective effect of Scoparia dulcis plant extract against Parkinson’s model of excitotoxicity in rats and zebrafish model. 18(4). 352–368.
Ayaz, M., Sadiq, A., Junaid, M., Ullah, F., Ovais, M., Ullah, I., ... & Shahid, M. (2019). Flavonoids as prospective neuroprotectants and their therapeutic propensity in aging associated neurological disorders. Frontiers in aging neuroscience, 11, 155.
Wang-Fischer, Y., & Koetzner, L. (2008). Tissue staining techniques for stroke studies. In Manual of Stroke Models in Rats (223–250). CRC Press
Misra, H. P., & Fridovich, I. (1977). Superoxide dismutase:“positive” spectrophotometric assays. Analytical biochemistry, 79(1–2), 553–560.
doi: 10.1016/0003-2697(77)90429-8
pubmed: 17332
Aebi, H. (1974). Catalase. In Methods of enzymatic analysis (673–684). Academic press.
Beauchamp, C. and Fridovich, I. (1971) Analyst. Bio- them. 44, 276-2.
Sun, J., Zhang, X., Broderick, M., & Fein, H. (2003). Measurement of nitric oxide production in biological systems by using Griess reaction assay. Sensors, 3(8), 276–284.
doi: 10.3390/s30800276
Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., & Hoekstra, W. (1973). Selenium: Biochemical role as a component of glutathione peroxidase. Science, 179(4073), 588–590.
doi: 10.1126/science.179.4073.588
pubmed: 4686466
Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A. G., ... & Stadtman, E. R. (1990). [49] Determination of carbonyl content in oxidatively modified proteins. In Methods in enzymology (186, 464–478). Academic Press.
Chomczynski, P., & Sacchi, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical biochemistry, 162(1), 156–159.
doi: 10.1016/0003-2697(87)90021-2
pubmed: 2440339
Pennisi, M., Malaguarnera, G., Puglisi, V., Vinciguerra, L., Vacante, M., & Malaguarnera, M. (2013). Neurotoxicity of acrylamide in exposed workers. International journal of environmental research and public health, 10(9), 3843–3854.
doi: 10.3390/ijerph10093843
pubmed: 23985770
pmcid: 3799507
Amirshahrokhi, K., & Abzirakan, A. (2022). Carvedilol attenuates acrylamide-induced brain damage through inhibition of oxidative, inflammatory, and apoptotic mediators. Iranian Journal of Basic Medical Sciences, 25(1), 60.
pubmed: 35656449
pmcid: 9118273
Farhana A, Lappin SL (2022). Biochemistry, lactate dehydrogenase. In: StatPearls. Treasure Island (FL): StatPearls Publishing.
Picón-Pagès, P., Garcia-Buendia, J., & Muñoz, F. J. (2019). Functions and dysfunctions of nitric oxide in brain. Biochimica et biophysica acta. Molecular basis of disease, 1865(8), 1949–1967.
doi: 10.1016/j.bbadis.2018.11.007
pubmed: 30500433
Aoyama, K. (2021). Glutathione in the brain. International journal of molecular sciences, 22(9), 5010.
doi: 10.3390/ijms22095010
pubmed: 34065042
pmcid: 8125908
Li, Y. P., Wu, D. X., Ye, T., & Zhang, H. (2022). Cytotoxic diterpenoids from the aerial parts of Scoparia dulcis. Phytochemistry Letters, 49, 21–26.
doi: 10.1016/j.phytol.2022.02.014
Haridevamuthu, B., Guru, A., Murugan, R., Sudhakaran, G., Pachaiappan, R., Almutairi, M. H., ... & Arockiaraj, J. (2022). Neuroprotective effect of biochanin A against bisphenol A-induced prenatal neurotoxicity in zebrafish by modulating oxidative stress and locomotory defects. Neuroscience Letters, 790 136889.