A biotechnological approach for the production of new protein bioplastics.
E. coli
biopolymer
disulfide bridges
phaseolin
transplastomic plants
Journal
Biotechnology journal
ISSN: 1860-7314
Titre abrégé: Biotechnol J
Pays: Germany
ID NLM: 101265833
Informations de publication
Date de publication:
06 Oct 2023
06 Oct 2023
Historique:
revised:
06
09
2023
received:
27
07
2023
accepted:
27
09
2023
pubmed:
6
10
2023
medline:
6
10
2023
entrez:
6
10
2023
Statut:
aheadofprint
Résumé
The future of biomaterial production will leverage biotechnology based on the domestication of cells as biological factories. Plants, algae, and bacteria can produce low-environmental impact biopolymers. Here, two strategies were developed to produce a biopolymer derived from a bioengineered vacuolar storage protein of the common bean (phaseolin; PHSL). The cys-added PHSL* forms linear-structured biopolymers when expressed in the thylakoids of transplastomic tobacco leaves by exploiting the formation of inter-chain disulfide bridges. The same protein without signal peptide (ΔPHSL*) accumulates in Escherichia coli inclusion bodies as high-molar-mass species polymers that can subsequently be oxidized to form disulfide crosslinking bridges in order to increase the stiffness of the biomaterial, a valid alternative to the use of chemical crosslinkers. The E. coli cells produced 300 times more engineered PHSL, measured as percentage of total soluble proteins, than transplastomic tobacco plants. Moreover, the thiol groups of cysteine allow the site-specific PEGylation of ΔPHSL*, which is a desirable functionality in the design of a protein-based drug carrier. In conclusion, ΔPHSL* expressed in E. coli has the potential to become an innovative biopolymer.
Identifiants
pubmed: 37801630
doi: 10.1002/biot.202300363
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300363Subventions
Organisme : European Union - NextGenerationEU under the Italian Ministry of University and Research (MUR) National Innovation Ecosystem
ID : ECS00000041 - VITALITY-CUPH33C22000430006
Informations de copyright
© 2023 The Authors. Biotechnology Journal published by Wiley-VCH GmbH.
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