Impaired decidualization and relative increase of PROK1 expression in the decidua of patients with unexplained recurrent pregnancy loss showing insulin resistance.
Pregnancy
Female
Humans
Decidua
/ pathology
Insulin-Like Growth Factor Binding Protein 1
/ metabolism
Insulin-Like Growth Factor II
/ metabolism
Insulin Resistance
Abortion, Habitual
/ pathology
Insulin
Gastrointestinal Hormones
/ metabolism
Vascular Endothelial Growth Factor, Endocrine-Gland-Derived
/ metabolism
Decidua
IGF-2
IGFBP-I
Insulin resistance
PROK1
Recurrent pregnancy loss
Journal
Journal of reproductive immunology
ISSN: 1872-7603
Titre abrégé: J Reprod Immunol
Pays: Ireland
ID NLM: 8001906
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
24
06
2023
revised:
27
08
2023
accepted:
20
09
2023
medline:
5
12
2023
pubmed:
7
10
2023
entrez:
6
10
2023
Statut:
ppublish
Résumé
A recent meta-analysis revealed that patients with unexplained recurrent pregnancy loss (RPL) show higher insulin resistance compared to healthy controls. However, the etiology of RPL remains unknown. Prokineticin (PROK1), a pleiotropic uterine endometrial protein, is important for implantation and decidualization and is regulated by hypoxia and insulin. In this study, we investigated the decidualization status and the role of PROK1 in the decidua of patients with unexplained RPL showing insulin resistance. Thirty-two patients with unexplained RPL were included in this study. Following the diagnosis of a miscarriage, the decidua and villi of the patient were surgically collected. Fasting blood glucose and insulin levels were measured, and HOMA-β was calculated. Using IHC and ELISA, the expression of IGFBP-1, PRL and PROK1 in the decidua and IGF-2 in the villi were analyzed in patients with euploid miscarriage with a high HOMA-β index (n = 8) and compared to controls (euploid miscarriage with normal HOMA-β: n = 12, aneuploid miscarriage with normal HOMA-β: n = 12). The co-localization of PROK1 and IGFBP-1 was observed in the decidua by IHC. In the decidua of RPL patients with high HOMA-β, the expression levels of IGFBP-1 and PRL were significantly lower, whereas the PROK1/IGFBP-1 ratio was significantly higher compared to that of the controls. IGF-2 expression in villi was significantly lower in RPL patients with high HOMA-β. Impaired decidualization and excessive PROK1 production may have pathological implications in patients with unexplained RPL with insulin resistance, especially under the state of hyper insulin production.
Identifiants
pubmed: 37801889
pii: S0165-0378(23)00361-3
doi: 10.1016/j.jri.2023.104155
pii:
doi:
Substances chimiques
Insulin-Like Growth Factor Binding Protein 1
0
Insulin-Like Growth Factor II
67763-97-7
Insulin
0
PROK1 protein, human
0
Gastrointestinal Hormones
0
Vascular Endothelial Growth Factor, Endocrine-Gland-Derived
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104155Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.