Cooperative CAR targeting to selectively eliminate AML and minimize escape.

CAR T cell therapy ICAHT IF-BETTER gate acute myeloid leukemia chimeric antigen receptor combinatorial targeting cooperative CAR hematotoxicity off-target toxicity on-target toxicity

Journal

Cancer cell
ISSN: 1878-3686
Titre abrégé: Cancer Cell
Pays: United States
ID NLM: 101130617

Informations de publication

Date de publication:
13 11 2023
Historique:
received: 22 03 2023
revised: 20 07 2023
accepted: 15 09 2023
medline: 16 11 2023
pubmed: 7 10 2023
entrez: 6 10 2023
Statut: ppublish

Résumé

Acute myeloid leukemia (AML) poses a singular challenge for chimeric antigen receptor (CAR) therapy owing to its phenotypic heterogeneity and similarity to normal hematopoietic stem/progenitor cells (HSPCs). Here we expound a CAR strategy intended to efficiently target AML while minimizing HSPC toxicity. Quantification of target expression in relapsed/refractory patient samples and normal HSPCs reveals a therapeutic window for gated co-targeting of ADGRE2 and CLEC12A: We combine an attenuated ADGRE2-CAR with a CLEC12A-chimeric costimulatory receptor (ADCLEC.syn1) to preferentially engage ADGRE2

Identifiants

pubmed: 37802054
pii: S1535-6108(23)00326-4
doi: 10.1016/j.ccell.2023.09.010
pii:
doi:

Substances chimiques

CLEC12A protein, human 0
Receptors, Mitogen 0
Lectins, C-Type 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1871-1891.e6

Subventions

Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA254838
Pays : United States

Commentaires et corrections

Type : CommentIn

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests Memorial Sloan Kettering has submitted a patent application (WO2022232016A2) based in part on results presented in this manuscript (M.Sa., S.H., and J.M.-S. are listed among the inventors). M.Sa. and S.H. report research support and research funding from Takeda Pharmaceuticals related to the present research. M.Sa. reports research funding from Atara Biotherapeutics, Fate Therapeutics, and Mnemo Therapeutics unrelated to the present research. M.Sa. and I.R. are scientific cofounders of Mnemo Therapeutics. I.R. reports research funding from Atara Biotherapeutics, Takeda Pharmaceuticals; ownership/equity interests at Fate Therapeutics and Mnemo Therapeutics; intellectual property rights at Juno Therapeutics. K.F., M.R.N., and I.R. report employment at Takeda Pharmaceuticals. M.Su. declares the following competing interests: Novartis: Consultancy, Research Funding; Janssen: Consultancy; Seattle Genetics: Research Funding; Amgen: Consultancy, Honoraria, Research Funding; Celgene: Consultancy, Honoraria; Kite/Gilead: Consultancy, Honoraria, Research Funding; Roche AG: Consultancy, Research Funding. J.H.P. declares the following competing interest: research funding support from Takeda Pharmaceuticals, Fate Therapeutics, Genentech, InCyte and Servier; Consultancy from Amgen, Autolus, BMS, Curocel, Kite, Legend Biotech, Minerva, Pfizer, Servier, Sobi, and Takeda Pharmaceuticals; and serves on Scientific Advisory Board of Allogene, Artiva Biotherapeutics, and GC Cell Corporation.

Auteurs

Sascha Haubner (S)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Jorge Mansilla-Soto (J)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Sarah Nataraj (S)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Friederike Kogel (F)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Qing Chang (Q)

Antitumor Assessment Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Elisa de Stanchina (E)

Antitumor Assessment Core Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Molecular Pharmacology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Michael Lopez (M)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Mei Rosa Ng (MR)

Takeda Development Center Americas, Inc., Lexington, MA 02421, USA.

Kathryn Fraser (K)

Takeda Development Center Americas, Inc., Lexington, MA 02421, USA.

Marion Subklewe (M)

Department of Medicine III, University Hospital, LMU Munich, 81377 Munich, Germany.

Jae H Park (JH)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Cellular Therapy Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Xiuyan Wang (X)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Molecular Pharmacology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Michael G. Harris Cell Therapy and Cell Engineering Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Isabelle Rivière (I)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Molecular Pharmacology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA; Michael G. Harris Cell Therapy and Cell Engineering Facility, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.

Michel Sadelain (M)

Center for Cell Engineering, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. Electronic address: m-sadelain@ski.mskcc.org.

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Classifications MeSH