An investigation into the cytotoxic effects of microbubbles and their constituents on osteosarcoma and bone marrow stromal cells.
Bone
Microbubble
Phospholipids
Polyethylene glycol
Toxicity
Journal
Biochimica et biophysica acta. General subjects
ISSN: 1872-8006
Titre abrégé: Biochim Biophys Acta Gen Subj
Pays: Netherlands
ID NLM: 101731726
Informations de publication
Date de publication:
12 2023
12 2023
Historique:
received:
19
07
2023
revised:
26
09
2023
accepted:
02
10
2023
medline:
6
11
2023
pubmed:
7
10
2023
entrez:
6
10
2023
Statut:
ppublish
Résumé
Ultrasound-responsive microbubbles offer a means of achieving minimally invasive, localised drug delivery in applications including regenerative medicine. To facilitate their use, however, it is important to determine any cytotoxic effects they or their constituents may have. The aim of this study was to test the hypothesis that phospholipid-shelled microbubbles are non-toxic to human bone-derived cells at biologically-relevant concentrations. Microbubbles were fabricated using combinations of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dibehenoyl-sn-glycero-3-phosphocholine (DBPC), polyoxyethylene(40) stearate (PEG40S) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene-glycol)-2000] (DSPE-PEG DBPC:DSPE-PEG These data indicate that, over a broad range of concentrations and incubation times, DBPC:DSPE-PEG
Sections du résumé
BACKGROUND
Ultrasound-responsive microbubbles offer a means of achieving minimally invasive, localised drug delivery in applications including regenerative medicine. To facilitate their use, however, it is important to determine any cytotoxic effects they or their constituents may have. The aim of this study was to test the hypothesis that phospholipid-shelled microbubbles are non-toxic to human bone-derived cells at biologically-relevant concentrations.
METHODS
Microbubbles were fabricated using combinations of 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dibehenoyl-sn-glycero-3-phosphocholine (DBPC), polyoxyethylene(40) stearate (PEG40S) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene-glycol)-2000] (DSPE-PEG
RESULTS
DBPC:DSPE-PEG
CONCLUSIONS
These data indicate that, over a broad range of concentrations and incubation times, DBPC:DSPE-PEG
Identifiants
pubmed: 37802372
pii: S0304-4165(23)00179-4
doi: 10.1016/j.bbagen.2023.130481
pii:
doi:
Substances chimiques
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000)
0
Phospholipids
0
Antineoplastic Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
130481Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nicholas Evans reports financial support was provided by Engineering and Physical Sciences Research Council. Anastasia Polydorou reports financial support was provided by Engineering and Physical Sciences Research Council. Jonathan May reports financial support was provided by Engineering and Physical Sciences Research Council. Sara Ferri reports financial support was provided by Engineering and Physical Sciences Research Council. Qiang Wu reports financial support was provided by Engineering and Physical Sciences Research Council. Eleanor Stride reports financial support was provided by Engineering and Physical Sciences Research Council. Dario Carugo reports financial support was provided by Engineering and Physical Sciences Research Council.