The renoprotective effects of taurine against diabetic nephropathy via the p38 MAPK and TGF-β/Smad2/3 signaling pathways.
Rats
Animals
Diabetic Nephropathies
/ drug therapy
Rats, Wistar
Diabetes Mellitus, Experimental
/ pathology
Taurine
/ pharmacology
p38 Mitogen-Activated Protein Kinases
/ genetics
Kidney
/ metabolism
Signal Transduction
Oxidative Stress
Transforming Growth Factor beta
/ genetics
RNA, Messenger
/ metabolism
Extracellular Matrix Proteins
/ metabolism
Diabetic nephropathy
NOX4
Oxidative stress
Smad2/3
Taurine
p38 MAPK
Journal
Amino acids
ISSN: 1438-2199
Titre abrégé: Amino Acids
Pays: Austria
ID NLM: 9200312
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
24
01
2023
accepted:
20
09
2023
medline:
4
12
2023
pubmed:
8
10
2023
entrez:
7
10
2023
Statut:
ppublish
Résumé
Diabetic nephropathy (DN), a severe diabetes complication, causes kidney morphological and structural changes due to extracellular matrix accumulation. This accumulation is caused mainly by oxidative stress. Semi-essential amino acid derivative taurine has powerful antioxidant and antifibrotic effects. The aim of this study was to investigate the renoprotective effects of taurine through its possible roles in oxidative stress, extracellular matrix proteins, and the signaling pathways associated with the accumulation of extracellular matrix proteins in DN rats. 29 Wistar albino rats were randomly separated into control, taurine, diabetes, and diabetes + taurine groups. Diabetes animals were injected 45 mg/kg streptozosine. Taurine is given by adding to drinking water as 1% (w/v). Urine, serum, and kidney tissue were collected from rats for biochemical and histological analysis after 12 weeks. According to the studies, taurine significantly reduces the levels of malondialdehyde (MDA), total oxidant status (TOS), and protein expression of NADPH oxidase 4 (NOX4) that increase in diabetic kidney tissue. Also, decreased superoxide dismutase (SOD) activity levels significantly increased with taurine in diabetic rats. Moreover, increased mRNA and protein levels of fibronectin decreased with taurine. The matrix metalloproteinase (MMP)-2 and MMP-9 activities and their mRNA levels increased significantly, and this increase was significantly summed with taurine. There was a decrease in mRNA expression of Extracellular matrix metalloproteinase inducer (EMMPRIN). Taurine significantly increased this decrease. Diabetes increased mRNA expressions of transforming growth factor (TGF)-β and Smad2/3. Taurine significantly reduced this induction. TGF-β protein expression, p38, and Smad2/3 activations were also inhibited, but taurine was suppressed significantly. All these findings indicate that taurine may be an effective practical strategy to prevent renal diabetic injury.
Identifiants
pubmed: 37805666
doi: 10.1007/s00726-023-03342-w
pii: 10.1007/s00726-023-03342-w
doi:
Substances chimiques
Taurine
1EQV5MLY3D
p38 Mitogen-Activated Protein Kinases
EC 2.7.11.24
Transforming Growth Factor beta
0
RNA, Messenger
0
Extracellular Matrix Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1665-1677Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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