On the regulation of human D-aspartate oxidase.
D-aspartate
flavooxidase
neurotransmission
pLG72
post-translational modification
protein-protein interaction
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
11 2023
11 2023
Historique:
revised:
14
09
2023
received:
29
05
2023
accepted:
03
10
2023
medline:
30
10
2023
pubmed:
8
10
2023
entrez:
8
10
2023
Statut:
ppublish
Résumé
The human flavoenzyme D-aspartate oxidase (hDASPO) controls the level of D-aspartate in the brain, a molecule acting as an agonist of NMDA receptors and modulator of AMPA and mGlu5 receptors. hDASPO-induced D-aspartate degradation prevents age-dependent deterioration of brain functions and is related to psychiatric disorders such as schizophrenia and autism. Notwithstanding this crucial role, less is known about hDASPO regulation. Here, we report that hDASPO is nitrosylated in vitro, while no evidence of sulfhydration and phosphorylation is apparent: nitrosylation affects the activity of the human flavoenzyme to a limited extent. Furthermore, hDASPO interacts with the primate-specific protein pLG72 (a well-known negative chaperone of D-amino acid oxidase, the enzyme deputed to D-serine degradation in the human brain), yielding a ~114 kDa complex, with a micromolar dissociation constant, promoting the flavoenzyme inactivation. At the cellular level, pLG72 and hDASPO generate a cytosolic complex: the expression of pLG72 negatively affects the hDASPO level by reducing its half-life. We propose that pLG72 binding may represent a protective mechanism aimed at avoiding cytotoxicity due to H
Identifiants
pubmed: 37805834
doi: 10.1002/pro.4802
pmc: PMC10588558
doi:
Substances chimiques
Oxidoreductases
EC 1.-
D-Aspartate Oxidase
EC 1.4.3.1
D-Aspartic Acid
4SR0Q8YD1X
Carrier Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4802Informations de copyright
© 2023 The Authors. Protein Science published by Wiley Periodicals LLC on behalf of The Protein Society.
Références
J Pharm Biomed Anal. 2015 Dec 10;116:7-17
pubmed: 25868730
Biochim Biophys Acta. 1995 Feb 23;1243(2):283-6
pubmed: 7873575
Front Mol Biosci. 2021 May 10;8:684934
pubmed: 34041270
Neurobiol Aging. 2015 May;36(5):1890-902
pubmed: 25771393
Biomolecules. 2022 Jun 20;12(6):
pubmed: 35740983
Cell Mol Life Sci. 2010 Jul;67(14):2387-404
pubmed: 20195697
FEBS Lett. 2017 Feb;591(4):646-655
pubmed: 28166363
Amino Acids. 2012 Nov;43(5):1861-71
pubmed: 22851050
Protein Sci. 2023 Nov;32(11):e4802
pubmed: 37805834
Front Mol Biosci. 2019 Nov 12;6:125
pubmed: 31799256
FASEB J. 2020 Jan;34(1):1182-1197
pubmed: 31914658
Transl Psychiatry. 2015 Feb 17;5:e512
pubmed: 25689573
Neuroscience. 2000;100(1):183-9
pubmed: 10996468
Neurosci Lett. 2006 Feb 20;394(3):163-7
pubmed: 16298487
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 1;879(29):3108-21
pubmed: 21524944
Biochim Biophys Acta Proteins Proteom. 2018 Jul;1866(7):813-821
pubmed: 29410194
Front Mol Biosci. 2018 Jan 24;5:3
pubmed: 29417050
J Neurosci. 1996 Feb 1;16(3):1066-71
pubmed: 8558235
J Biol Chem. 2008 Aug 8;283(32):22244-56
pubmed: 18544534
FEBS J. 2023 Aug;290(15):3877-3895
pubmed: 37012601
Neuroscience. 1988 Jul;26(1):17-31
pubmed: 2901691
Protein Expr Purif. 2006 Mar;46(1):150-5
pubmed: 16183301
Front Mol Biosci. 2018 Nov 28;5:107
pubmed: 30547037
Cell Mol Life Sci. 2016 May;73(10):2029-39
pubmed: 26914235
Brain Res. 1998 Oct 12;808(1):65-71
pubmed: 9795139
Sci Rep. 2017 Apr 10;7:46288
pubmed: 28393897
NPJ Schizophr. 2017 Apr 6;3:16
pubmed: 28560262
Protein Sci. 2022 Jan;31(1):37-46
pubmed: 34216059
Mol Cell Neurosci. 2011 Sep;48(1):20-8
pubmed: 21679769
FEBS J. 2021 Aug;288(16):4939-4954
pubmed: 33650155
Chem Biodivers. 2010 Jun;7(6):1435-49
pubmed: 20564562
FEBS J. 2014 Feb;281(3):708-23
pubmed: 24237903
Mol Cell Neurosci. 2008 Feb;37(2):236-46
pubmed: 17981050
Neurosci Lett. 2010 Jul 12;478(3):128-30
pubmed: 20452401
J Neurosci. 2008 Oct 8;28(41):10404-14
pubmed: 18842900
Front Mol Biosci. 2017 Dec 15;4:88
pubmed: 29326945
Methods Enzymol. 2015;555:79-90
pubmed: 25747476
Neurobiol Aging. 2011 Dec;32(12):2229-43
pubmed: 20097447
J Biol Chem. 2013 Sep 13;288(37):26473-9
pubmed: 23861393
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13675-80
pubmed: 12364586
Front Mol Biosci. 2021 Jun 23;8:689719
pubmed: 34250021
J Neurosci. 2016 Mar 9;36(10):3064-78
pubmed: 26961959
Exp Neurol. 2019 Jul;317:51-65
pubmed: 30822420
Neurobiol Aging. 2011 Nov;32(11):2061-74
pubmed: 20031274
Protein Sci. 2006 Dec;15(12):2708-17
pubmed: 17088322