Cationic exchange SPE combined with triple quadrupole UHPLC-MS/MS for detection of GHRHs in urine samples.

Tandem Mass Spectrometry / methods Chromatography, High Pressure Liquid / methods Sermorelin Reproducibility of Results Solid Phase Extraction / methods
Growth hormone-releasing hormones (GHRHs) Solid-phase extraction Triple quadrupole UHPLC/MS-MS Weak cationic exchanger

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
01 12 2023
Historique:
received: 30 07 2023
revised: 19 09 2023
accepted: 28 09 2023
medline: 30 10 2023
pubmed: 9 10 2023
entrez: 8 10 2023
Statut: ppublish

Résumé

The use of growth hormone-releasing hormones (GHRHs) is prohibited in sports according to the regulations of the World Anti-Doping Agency (WADA). Considering the complexity of urine samples and the low concentrations at which these analytes should be detected, analyzing GHRHs is a challenging task. In most of the studies, GHRHs are analyzed using UHPLC-HRMS with an orbitrap. The present developed and validated method for some GHRHs (tesamorelin, CJC-1295, sermorelin (GRF 1-29), sermorelin (3-29)-NH

Identifiants

DOI: 10.1016/j.ab.2023.115336 PMID: 37806509
pubmed: 37806509
pii: S0003-2697(23)00301-9
doi: 10.1016/j.ab.2023.115336
pii:
doi:

Substances chimiques

Sermorelin 86168-78-7

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

115336

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Cătălina-Diana Cristea (CD)

University of Bucharest, Faculty of Chemistry, Department of Analytical and Physical Chemistry, Sos. Panduri, No. 90, Sect 5, Bucharest, 050663, Romania; Romanian Doping Control Laboratory, Bldv. Basarabia, No. 37-39, Sect 2, Bucharest, 022103, Romania. Electronic address: c.cristea@lcd.gov.ro.

Mihai Radu (M)

Romanian Doping Control Laboratory, Bldv. Basarabia, No. 37-39, Sect 2, Bucharest, 022103, Romania. Electronic address: m.radu@lcd.gov.ro.

Ani Toboc (A)

Romanian Doping Control Laboratory, Bldv. Basarabia, No. 37-39, Sect 2, Bucharest, 022103, Romania. Electronic address: a.toboc@lcd.gov.ro.

Cristina Stan (C)

Romanian Doping Control Laboratory, Bldv. Basarabia, No. 37-39, Sect 2, Bucharest, 022103, Romania. Electronic address: c.stan@lcd.gov.ro.

Victor David (V)

University of Bucharest, Faculty of Chemistry, Department of Analytical and Physical Chemistry, Sos. Panduri, No. 90, Sect 5, Bucharest, 050663, Romania. Electronic address: victor.david@chimie.unibuc.ro.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Cationic exchange SPE combined with triple...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Cationic exchange SPE combined with triple...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de tissu nerveux › Neuropeptides › Hormones hypothalamiques › Hormones hypophysiotropes libératrices › Hormone de libération de l'hormone de croissance › Sermoréline › Cationic exchange SPE combined with triple...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Cationic exchange SPE combined with triple...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Fractionnement chimique › Extraction en phase solide › Cationic exchange SPE combined with triple...