Polyubiquitin protein of Aedes aegypti as an interacting partner of dengue virus envelope protein.
Aedes aegypti
DENV (dengue virus)
polyubiquitin
protein-protein interaction
yeast two-hybrid assay
Journal
Medical and veterinary entomology
ISSN: 1365-2915
Titre abrégé: Med Vet Entomol
Pays: England
ID NLM: 8708682
Informations de publication
Date de publication:
08 Oct 2023
08 Oct 2023
Historique:
received:
06
06
2023
accepted:
07
09
2023
medline:
9
10
2023
pubmed:
9
10
2023
entrez:
9
10
2023
Statut:
aheadofprint
Résumé
Dengue virus (DENV) is an arbovirus that comprises four antigenically different serotypes. Aedes aegypti (Diptera: Culicidae) acts as the principal vector for DENV transmission, and vector control is crucial for dengue fever epidemic management. To design effective vector control strategies, a comprehensive understanding of the insect vector and virus interaction is required. Female Ae. aegypti ingests DENV during the acquisition of a blood meal from an infected human. DENV enters the insect midgut, replicates inside it and reaches the salivary gland for transmitting DENV to healthy humans during the subsequent feeding cycles. DENV must interact with the proteins present in the midgut and salivary glands to gain entry and accomplish successful replication and transmission. Ae. aegypti midgut cDNA library was prepared, and yeast two-hybrid screening was performed against the envelope protein domain III (EDIII) protein of DENV-2. The polyubiquitin protein was selected from the various candidate proteins for subsequent analysis. Polyubiquitin gene was amplified, and the protein was purified in a heterologous expression system for in vitro interaction studies. In vitro pull-down assay presented a clear interaction between polyubiquitin protein and EDIII. To further confirm this interaction, a dot blot assay was employed, and polyubiquitin protein was found to interact with DENV particles. Our results enable us to suggest that polyubiquitin plays an important role in DENV infection within mosquitoes.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Council of Scientific and Industrial Research (CSIR), India
ID : 09/045(1489)/2017-EMR-I
Organisme : Department of Science and Technology Science and Engineering Research Board (DST-SERB)
ID : EMR/2016/002636
Organisme : DST-PURSE
Informations de copyright
© 2023 Royal Entomological Society.
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